Diphenyleneiodonium, an NAD(P)H oxidase inhibitor, also potently inhibits mitochondrial reactive oxygen species production
Diphenyleneiodonium, an NAD(P)H oxidase inhibitor, also potently inhibits mitochondrial reactive oxygen species production
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DOI：
10.1006/bbrc.1998.9729
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发表时间：
1998-12-18
影响因子：
3.1
通讯作者：
Trush, MA
中科院分区：
文献类型：
--
作者：
Li, YB;Trush, MA
二苯基碘(DPI)经常被用于抑制黄素酶，特别是NAD(P)H氧化酶介导的活性氧(ROS)的产生。这项研究是为了检测DPI是否也能抑制线粒体产生超氧化物歧化酶和过氧化氢，线粒体是细胞内ROS的主要来源。未经刺激的单核/巨噬细胞用光敏素衍生的化学发光法(CL)检测线粒体超氧化物歧化产物。结果表明，DPI在抑制NAD(P)H氧化酶的浓度时可显著降低线粒体呼吸产生的超氧化物歧化产物。同样，在辣根过氧化物酶存在下，鲁米诺衍生的化学发光检测到线粒体呼吸产生的胞外过氧化氢也被DPI大大减少。DPI在抑制线粒体呼吸产生超氧化物和过氧化氢方面与鱼藤酮一样有效。在底物支持的分离线粒体中，DPI可能通过抑制NADH-泛醌氧化还原酶(复合体I)来减少线粒体超氧化物的产生。(C)1998年学术出版社。
Diphenyleneiodonium (DPI) has frequently been used to inhibit reactive oxygen species (ROS) production mediated by flavoenzymes, particularly NAD(P)H oxidase. This study was undertaken to examine if DPI could also inhibit production of superoxide and H2O2 by mitochondria, the major source of cellular ROS. Detection of mitochondrial superoxide by lucigenin-derived chemiluminescence (CL) with unstimulated monocytes/macrophages showed that DPI at concentrations that inhibit NAD(P)H oxidase markedly diminished the production of superoxide by mitochondrial respiration. Similarly, the extracellular H2O2 derived from mitochondrial respiration as detected by luminol-derived CL in the presence of horseradish peroxidase was also greatly reduced by DPI. DPI was as potent as rotenone in inhibiting the production of superoxide and H2O2 by mitochondrial respiration. With substrate-supported isolated mitochondria, DPI was shown to reduce mitochondrial superoxide production probably through inhibiting NADH-ubiquinone oxidoreductase (complex I). (C) 1998 Academic Press.