SYNAPTIC TRANSMISSION BETWEEN RAT CEREBELLAR GRANULE AND PURKINJE-CELLS IN DISSOCIATED CELL-CULTURE - EFFECTS OF EXCITATORY-AMINO ACID TRANSMITTER ANTAGONISTS
SYNAPTIC TRANSMISSION BETWEEN RAT CEREBELLAR GRANULE AND PURKINJE-CELLS IN DISSOCIATED CELL-CULTURE - EFFECTS OF EXCITATORY-AMINO ACID TRANSMITTER ANTAGONISTS
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DOI：
10.1073/pnas.85.3.934
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发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
HAGIWARA, S
中科院分区：
文献类型：
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作者：
HIRANO, T;HAGIWARA, S
在解离细胞培养物中研究了小脑颗粒和浦肯野细胞之间的单突触兴奋性连接，并对该突触处的递质和突触后受体的识别进行了药理学研究。同时对突触前颗粒细胞和突触后浦肯野细胞进行电压或电流钳位，并检查颗粒细胞诱导的兴奋性突触后电流。正如我们早期的研究一样，神经元和单突触兴奋性连接被识别。几对颗粒细胞和浦肯野细胞分别用荧光黄和碘化丙啶染色，并在电生理记录后检查继承形态。单突触兴奋性突触后电流被 1 mM 犬尿酸盐（一种兴奋性氨基酸受体拮抗剂）抑制，但受到 0.2 mM DL-2-氨基-5-磷酸新戊酸盐（N-甲基-D-天冬氨酸受体选择性拮抗剂）的影响很小。谷氨酸和天冬氨酸在浦肯野细胞中诱导内向电流。这些电流被 1 mM 的犬尿酸盐抑制。 0.2mM的DL-2-氨基-5-磷酸戊酸盐抑制由100μM天冬氨酸诱导的内向电流，但不影响由10μM谷氨酸诱导的内向电流。这些结果与以下观点一致：谷氨酸或谷氨酸样物质，但不是天冬氨酸，是颗粒细胞和浦肯野细胞之间的突触处释放的递质，并且非 N-甲基-D-天冬氨酸受体通道在突触后膜中发挥作用。
Monosynaptic excitatory connections between cerebellar granule and Purkinje cells were studied in dissociation cell cultures, and identification of the transmitter and the postsynaptic receptor at this synapse was pharmacologically investigated. The presynaptic granule cell and the postsynaptic Purkinje cell were voltage- or current-clamped simultaneously and the excitatory postsynaptic current induced by the granule cell was examined. The neurons and monosynaptic excitatory connections were identified as in our earlier study. Several pairs of granule and Purkinje cells were stained with Lucifer yellow and propidium iodide, respectively, and heir morphology was examined after electrophysiological recording. The monosynaptic excitatory postsynaptic current was suppressed by 1 mM kynurenate, an antagonist for excitatory-amino acid receptors, but was little affected by 0.2 mM DL-2-amino-5-phosphonovalerate, a selective antagonist of N-methyl-D-aspartate receptors. Glutamate and aspartate induced inward current in the Purkinje cells. These currents were suppressed by kynurenate at 1 mM. DL-2-Amino-5-phosphonovalerate at 0.2 mM suppressed the inward current induced by 100 .mu.M aspartate but did not affect the inward current induced by 10 .mu.M glutamate. These results are consistent with the idea that glutamate, or a glutamate-like substance, but not aspartate is the transmitter released at the synapse between granule and Purkinje cells and that non-N-methyl-D-aspartate receptor channels are functioning in the postsynaptic membrane.