Lactobacillus casei DN-114 001 inhibits the increase in paracellular permeability of enteropathogenic Escherichia coli-infected T84 cells
Lactobacillus casei DN-114 001 inhibits the increase in paracellular permeability of enteropathogenic Escherichia coli-infected T84 cells
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DOI：
10.1016/j.resmic.2004.09.013
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发表时间：
2005-03-01
影响因子：
2.6
通讯作者：
Rampal, P
中科院分区：
文献类型：
--
作者：
Parassol, N;Freitas, M;Rampal, P
益生菌是活的微生物，当摄入足够的量时，对宿主产生健康益处。例如，益生菌可能通过增强肠上皮屏障功能发挥作用。本研究的目的是确定干酪乳杆菌DN-114 001是否可以消除肠致病性大肠杆菌引起的细胞旁通透性增加。我们使用感染野生型肠致病性大肠杆菌的人结肠T84细胞系。coli（菌株E2348/69）。通过监测跨上皮电阻变化和观察闭合小带-1的分布来跟踪细胞旁通透性。使用两种感染程序：共孵育（致病菌株和益生菌菌株与T84细胞同时孵育）和感染后（在感染开始后3小时在致病菌存在下添加益生菌）。我们还研究了L. casei对肠致病性E.大肠杆菌粘附。L. casei DN-114 001以剂量依赖性方式抑制肠致病性E.大肠杆菌诱导的跨上皮电阻和闭锁小带-1重新分布使用两种不同的感染程序。然而，L.干酪素不抑制致病菌株的粘附。L.酪蛋白DN-114 001抑制EPEC诱导的细胞旁通透性的增加。这种特性可以部分解释这种益生菌对人类自然防御的健康益处，例如与预防腹泻有关的益处。(c)2004年，Elsevier SAS。All rights reserved.
Probiotics are living microorganisms which, when ingested in adequate amounts, exert health benefits toward the host. For instance, probiotics might act through reinforcement of the intestinal epithelial barrier function. The goal of the present study was to determine whether Lactobacillus casei DN-114 001 could abrogate the increase in paracellular permeability induced by enteropathogenic Escherichia coli. We used the human colon T84 cell line infected with a wild-type enteropathogenic E. coli (strain E2348/69). Paracellular permeability was followed by monitoring transepithelial electrical resistance variations and by observing zonula occludens-1 distribution. Two infection procedures were used: co-incubation (the pathogenic and probiotic strains were simultaneously incubated with T84 cells) and post-infection (the probiotic was added in the presence of pathogenic bacteria 3 h after the beginning of the infection). We also investigated the effect of L. casei on enteropathogenic E. coli adhesion. L. casei DN-114 001 inhibited, in a dose-dependent-manner, the decrease in enteropathogenic E. coli-induced transepithelial electrical resistance and zonula occludens-1 redistribution using two different infection procedures. However, L. casei did not inhibit pathogenic strain adhesion. L. casei DN-114 001 inhibited the increase in EPEC-induced paracellular permeability. This property could partially explain the previously observed health benefits of this probiotic for human natural defenses, such as those associated with prevention of diarrhea. (c) 2004 Elsevier SAS. All rights reserved.