A novel regulation of the oviductal glycoprotein gene expression by luteinizing hormone in bovine tubal epithelial cells.
A novel regulation of the oviductal glycoprotein gene expression by luteinizing hormone in bovine tubal epithelial cells.
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黄体生成素对牛输卵管上皮细胞中输卵管糖蛋白基因表达的新调节。
DOI：
10.1016/s0303-7207(97)00104-4
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发表时间：
1997
影响因子：
4.1
通讯作者：
Rao,CV
中科院分区：
文献类型：
--
作者：
Sun,T;Lei,ZM;Rao,CV
本文验证了牛输卵管上皮细胞含有促黄体生成素(LH)/人绒毛膜促性腺激素（hCG）受体，LH可能调控输卵管糖蛋白（OGP）基因表达的假说。结果表明，牛输卵管上皮细胞含有一个80 kDa的LH/hCG受体蛋白，可结合[125I]hCG。用高纯度hCG（作为LH的替代激素）培养这些细胞，导致95 kDa蛋白和2.3 kb的OGP转录物的剂量和时间依赖性增加。当hCG浓度为10 ng/ml时，培养3天后，细胞增殖率达到100%。LH，而不是糖蛋白激素家族中的其他激素，hCG的分离亚基，雌二醇-17β或黄体酮，模仿hCG，这表明这种作用是激素特异性的，需要天然激素的构象。hCG增加稳态OGP mRNA水平不是由于基因转录率的增加。这是由于OGP转录本的半衰期从对照组的23小时显著增加到hCG治疗后的28小时。综上所述，我们得出结论，LH和hCG可以通过降低牛输卵管上皮细胞中OGP转录本的降解来增加OGP的合成。由于OGP可能在早期胚胎的受精和生长发育中起着重要的作用，因此在排卵期存在较高的LH水平可能通过增加OGP的合成而直接参与了早期妊娠事件。
The hypothesis that bovine oviductal epithelial cells contain luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptors and LH may regulate the oviductal glycoprotein (OGP) gene expression was tested. The results showed that bovine oviductal epithelial cells contain an 80 kDa LH/hCG receptor protein which can bind [125I]hCG. Culturing these cells with highly purified hCG, used as a surrogate hormone for LH, resulted in a dose and time dependent increase in a 95 kDa protein and a 2.3 kb transcript of OGP. The maximal increase of 100% was obtained with 10 ng/ml hCG and after three days of culture. LH, but not other hormones in the glycoprotein hormone family, isolated subunits of hCG, estradiol-17β or progesterone, mimicked hCG, suggesting that the effect is hormone specific and requires the conformation of native hormone. The increase in steady state OGP mRNA levels by hCG is not due to an increase in the transcription rate of the gene. It is rather due to a significant increase in the half-life of OGP transcripts from 23 h in the control to 28 h after treatment with hCG. In summary, we conclude that LH and hCG can increase the synthesis of OGP by decreasing the degradation of its transcripts in bovine oviductal epithelial cells. Since OGP may play important roles in fertilization and growth and development of early embryo, higher LH levels present during the periovulatory period may directly participate in the early pregnancy events through increasing the synthesis of OGP.