TaFlo2-A1, an ortholog of rice Flo2, is associated with thousand grain weight in bread wheat (Triticum aestivum L.).
TaFlo2-A1, an ortholog of rice Flo2, is associated with thousand grain weight in bread wheat (Triticum aestivum L.).
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TaFlo2-A1 是水稻 Flo2 的直系同源物，与面包小麦 (Triticum aestivum L.) 的千粒重相关。
DOI：
10.1186/s12870-017-1114-3
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发表时间：
2017-10-16
影响因子：
5.3
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Sajjad M;Ma X;Habibullah Khan S;Shoaib M;Song Y;Yang W;Zhang A;Liu D
背景：Flo2基因是植物中一个保守基因家族的成员。该基因与水稻千粒重（TGW）有关。克隆了TaFlo2-A1在小麦六倍体中的同源物，并检测了TaFlo2-A1的单倍型变异与TGW的关联。结果：克隆的TaFlo2-A1、TaFlo2-B1和TaFlo2-D1分别含有23、23和24个外显子。TaFlo2-A1 （1734 aa）、TaFlo2-B1 （1698 aa）和TaFlo2-D1 （1682 aa）蛋白高度相似（>94%），与水稻FLO2蛋白相似度为>77%。与水稻FLO2蛋白一样，在推断的tflo2蛋白中观察到四个四肽重复（TPR）基序。在面包小麦中，启动子区一个8 bp的InDel （-10 ~ -17 bp）和TaFlo2-A1第一个内含子上的5个snp共同形成了TaFlo2-A1a和TaFlo2-A1b两个单倍型。TaFlo2位于同源组2染色体上。推断TaFlo2-A1位于删除bin ‘2AL1-0.85-1.00’上。利用分子标记TaFlo2-Indel8对中国小麦和巴基斯坦小麦的TaFlo2-A1单倍型进行了鉴定。在MCC和巴基斯坦小麦中，TaFlo2-A1被发现与总重有关，但与每穗粒数（GpS）无关。TaFlo2-A1b阳性单倍型在商品小麦品种中频率较低；因此，可以选择这种单倍型来提高粒重，而不会对全球定位产生负面影响。在携带阳性单倍型的品种中，TaFlo2-A1在开花后5daf （d后）发育的籽粒中表达量与TGW呈正相关。结论：本研究为进一步了解小麦六倍体中Flo2基因的调控机制奠定了基础。此外，新建立的分子标记“TaFlo2-InDel8”可纳入小麦育种者的标记辅助选择试剂盒中。
Background:The Flo2 gene is a member of a conserved gene family in plants. This gene has been found to be related to thousand grain weight (TGW) in rice. Its orthologs in hexaploid wheat were cloned, and the haplotype variation in TaFlo2-A1 was tested for association with TGW.Results:The cloned sequences of TaFlo2-A1, TaFlo2-B1 and TaFlo2-D1 contained 23, 23 and 24 exons, respectively. The deduced proteins of TaFlo2-A1 (1734 aa), TaFlo2-B1 (1698 aa) and TaFlo2-D1 (1682 aa) were highly similar (>94%) and exhibited >77% similarity with the rice FLO2 protein. Like the rice FLO2 protein, four tetratricopeptide repeat (TPR) motifs were observed in the deduced TaFLO2 protein. An 8-bp InDel (-10 to -17 bp) in the promoter region and five SNPs in first intron of TaFlo2-A1 together formed two haplotypes, TaFlo2-A1a and TaFlo2-A1b, in bread wheat. TaFlo2 was located on homeologous group 2 chromosomes. TaFlo2-A1 was inferred to be located on deletion bin '2AL1-0.85-1.00'. The TaFlo2-A1 haplotypes were characterized in the Chinese Micro Core Collection (MCC) and Pakistani wheat collection using the molecular marker TaFlo2-Indel8. TaFlo2-A1 was found to be associated with TGW but not with grain number per spike (GpS) in both the MCC and Pakistani wheat collections. The frequency of TaFlo2-A1b (positive haplotype) was low in commercial wheat cultivars; thus this haplotype can be selected to improve grain weight without negatively affecting GpS. The expression level of TaFlo2-A1 in developing grains at 5 DAF (days after flowering) was positively correlated with TGW in cultivars carrying the positive haplotype.Conclusion:This study will likely lead to additional investigations to understand the regulatory mechanism of the Flo2 gene in hexaploid wheat. Furthermore, the newly developed molecular marker 'TaFlo2-InDel8' could be incorporated into the kit of wheat breeders for use in marker-assisted selection.
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影响因子：
30.8
作者：
Li, Yibo;Fan, Chuchuan;Zhang, Qifa
通讯作者：
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影响因子：
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作者：
Sajjad, M.;Khan, S. H.;Khan, I. A.
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DOI：
10.1073/pnas.252763199
发表时间：
2003-03-04
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Edwards, K