Bilayer-domain formation of thermoresponsive amphiphilic block copolymers in hybrid liposomes for synthetic molecular channels
Bilayer-domain formation of thermoresponsive amphiphilic block copolymers in hybrid liposomes for synthetic molecular channels
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DOI：
10.1039/d3py00223c
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发表时间：
2023-04-20
影响因子：
4.6
通讯作者：
Nishimura，Tomoki
中科院分区：
文献类型：
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作者：
Ozawa，Naoki;Kosaka，Shunji;Nishimura，Tomoki
分子可渗透囊泡作为纳米反应器或人工细胞的隔室已经引起了人们的兴趣，因为它们可以允许分子从囊泡的外部运输到内部。增加囊泡渗透性的一种策略是将含有温敏性聚（环氧丙烷）的两亲性共聚物掺入磷脂囊泡中。然而，这些聚合物的纳米结构，这是直接相关的分子渗透性，仍然有待阐明。在这里，我们报告的纳米结构的聚合物纳入这样的膜和渗透性的聚合物/磷脂混合囊泡对水溶性分子。中子散射实验表明，该聚合物在膜上形成盘状双层结构域，其厚度几乎等于当聚合物单独自组装成囊泡时的双层膜的厚度。这些结构域充当低分子量聚乙二醇的合成分子通道，并且它们的渗透性可以通过改变聚环氧丙烷的分子量来调节。这些发现为合成通道的新型聚合物的设计、其渗透性的预测以及可渗透囊泡的制备提供了有价值的见解。
Molecularly permeable vesicles have attracted interest as compartments for nanoreactors or artificial cells, as they can allow the transport of molecules from the exterior to the interior of the vesicles. One strategy to increase vesicle permeability is the incorporation of amphiphilic copolymers that contain thermoresponsive poly(propylene oxide) into phospholipid vesicles. However, the nanostructures of these polymers, which are directly related to the molecular permeability, remain to be elucidated. Here, we report on the nanostructures of polymers incorporated in such membranes and the permeability of the polymer/phospholipid hybrid vesicles against water-soluble molecules. Neutron-scattering experiments revealed that the polymers form disk-like bilayer domains on the membranes, whose thickness is almost equivalent to that of the bilayer membranes when the polymers alone are self-assembled into vesicles. These domains act as synthetic molecular channels for low-molecular-weight poly(ethylene glycol), and their permeability can be regulated by changing the molecular weight of the poly(propylene oxide). These findings provide valuable insight into the design of novel polymers for synthetic channels, the prediction of their permeability, and the preparation of permeable vesicles.