Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?
Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?
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DOI：
10.1016/j.joca.2005.05.005
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发表时间：
2005-10-01
影响因子：
7
通讯作者：
Lee, KB
中科院分区：
文献类型：
--
作者：
Im, GI;Shin, YW;Lee, KB
目的：脂肪组织源性间充质干细胞（ATMSC）已被证明可以分化为骨、软骨、脂肪或肌肉。然而，尚不确定 ATMSC 与骨髓间充质干细胞 (BMMSC) 的骨和软骨形成潜力相同。本研究的目的就是回答这个问题。方法：从股骨髓管中获取BMMSCs，从吸脂手术中收集的脂肪中分离ATMSCs。细胞在培养基中扩增并传代两次后，进行 STRO-1 和 CD34 的免疫荧光研究以表征 BMMSC 和 ATMSC。用含有地塞米松、β-甘油磷酸和抗坏血酸的成骨培养基在单层培养物上诱导成骨。 2-3周后，进行碱性磷酸酶（AP）和Von Kossa染色。为了测试软骨形成，将间充质干细胞 (MSC) 在颗粒培养物和纤维蛋白支架中培养，并使用含有转化生长因子-β(2) 和胰岛素样生长因子-I 的软骨形成培养基 (CM)。 4周后，进行番红-O染色和II型胶原免疫组织化学染色，以评估软骨形成分化和基质产生。采用组织学标准半定量评估软骨形成程度。结果：BMMSCs和ATMSCs均为STRO-1阳性、CD34阴性。在成骨测试中，AP染色显示ATMSCs的成骨分化能力远低于BMMSCs（P = 0.002）。 Von Kossa 染色显示的基质矿化量也显示出两种 MSC 之间的统计差异（P = 0.011）。在通过颗粒培养物进行软骨形成测试时，ATMSC 在细胞形态和基质生成方面显示出软骨形成细胞的表现要弱得多。 BMMSCs的组织学评分为6.5（SD1.3），CM中培养的ATMSCs的组织学评分为4.3（SD1.6），具有统计学意义（P = 0.023）。纤维蛋白凝胶的结果与一般颗粒培养物的结果相似。结论：我们的研究结果表明，与 BMMSC 相比，ATMSC 的成骨和软骨形成潜力可能较差，这对脂肪组织作为 MSC 来源的价值产生了怀疑。 (c) 2005 年国际骨关节炎研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Adipose tissue-derived mesenchymal stem cells (ATMSCs) have been shown to differentiate into bone, cartilage, fat or muscle. However, it is not certain that ATMSCs are equal to bone marrow-derived mesenchymal stem cells (BMMSC) for their bone and cartilage forming potential. The purpose of this study was to answer the question.Methods: BMMSCs were obtained from the medullary canal of femur and ATMSCs were isolated from the fat harvested during liposuction procedures. After cell expansion in culture media and two passages, the immunofluorescent studies for STRO-1 and CD34 were performed to characterize the BMMSCs and ATMSCs. Osteogenesis was induced on a monolayer culture with osteogenic medium containing dexamethasone, beta-glycerophosphate and ascorbate. After 2-3 weeks, alkaline phosphatase (AP) and Von Kossa staining were done. To test for chondrogenesis, mesenchymal stem cells (MSCs) were cultured in a pellet culture and in a fibrin scaffold with a chondrogenic medium (CM) containing transforming growth factor-beta(2) and insulin-like growth factor-I. After 4 weeks, Safranin-O staining and immunohistochemical staining for type II collagen were done to evaluate the chondrogenic differentiation and the matrix production. A histological scale was used to semiquantitatively assess the degree of chondrogenesis.Results: Both BMMSCs and ATMSCs were STRO-1 positive and CD34 negative. On the test of osteogenesis, the osteoblastic differentiation of ATMSCs as demonstrated by AP staining was much less than that of the BMMSCs (P = 0.002). The amount of matrix mineralization shown by Von Kossa staining also showed statistical differences between the two MSCs (P = 0.011). On the test for chondrogenesis by the pellet culture ATMSCs showed much weaker presentation as chondrogenic cells in both cell morphology and the matrix production. The histological score was 6.5 (SD1.3) for the BMMSCs, and 4.3 (SD1.6) for the ATMSCs cultured in CM, which was statistically significant (P = 0.023). The results from fibrin gel paralleled those from the pellet culture in general.Conclusion: The results of our study suggest that the ATMSCs may have an inferior potential for both osteogenesis and chondrogenesis compared with the BMMSCs, and these cast doubts on the value of adipose tissue as a source of MSCs. (c) 2005 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.