Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States
Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States
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DOI：
10.1016/j.vetmic.2006.07.017
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发表时间：
2006-12-20
影响因子：
3.3
通讯作者：
Richt, Juergen A.
中科院分区：
文献类型：
--
作者：
Vincent, Amy L.;Lager, Kelly M.;Richt, Juergen A.
从美国（U.S.）分离的血凝素亚型I（III）的猪流感病毒（SIV）在自然宿主中还没有得到很好的表征。自1998年以来，来自美国的SIV分离株（包括属于H1亚型的病毒）的突变和重配率有所增加。进行了两项独立的动物研究，以评价和比较10个SIV分离株的发病机制，这些分离株可追溯到1930年，目前正在流行。此外，对每个分离株的血凝素和神经氨酸酶基因进行测序以进行遗传比较，并使用血凝抑制和血清中和试验中的所有血清和病毒组合评估血清学交叉反应性。在分离株之间检测到肺炎百分比和肺中病毒滴度的统计学显著差异，现代分离株倾向于产生更严重的疾病，具有更多的病毒脱落和更高的病毒滴度。然而，鼻脱落和肺中的病毒滴度并不总是彼此相关或与肺部病变相关。在血清学上，经典的历史H1N1病毒倾向于在历史血清和抗原之间具有更好的交叉反应，与现代病毒抗原具有中度至良好的交叉反应性。然而，现代血清与历史病毒的反应性较低。现代病毒倾向于在现代组内具有较不一致的交叉反应性。总体而言，在过去75年中从美国猪群中收集的H1分离株在致病性方面表现出相当大的变异性。1998年，随着猪三重耐药株H3N2的出现，遗传和抗原多样性似乎有所增加。(c)2006 Elsevier B.V.保留所有权利。
Swine influenza viruses (SIV) of the hemagglutinin subtype I (III) isolated from the United States (U.S.) have not been well-characterized in the natural host. An increase in the rate of mutation and reassortment has occurred in SIV isolates from the U.S. since 1998, including viruses belonging to the H1 subtype. Two independent animal studies were done to evaluate and compare the pathogenesis of 10 SIV isolates dating from 1930 to currently circulating isolates. In addition, the hemagglutinin and neuraminidase genes of each isolate were sequenced for genetic comparison, and serological cross-reactivity was evaluated using all sera and virus combinations in hemagglutination inhibition and serum neutralization assays. Statistically significant differences in percentage of pneumonia and virus titers in the lung were detected between isolates, with modern isolates tending to produce more severe disease, have more virus shedding and higher viral titers. However, nasal shedding and virus titers in the lung were not always correlated with one another or lung lesions. Serologically, the classic historical H1N1 viruses tended to have better cross-reaction between historical sera and antigens, with moderate to good cross-reactivity with modern viral antigens. However, the modern sera were less reactive with historical viruses. Modern viruses tended to have less consistent cross-reactivity within the modern group. Overall, H1 isolates collected over the last 75 years from the U.S. pig population exhibit considerable variability in pathogenicity. There appears to be an increase in genetic and antigenic diversity coincident with the emergence of the swine triple reassortant H3N2 in 1998. (c) 2006 Elsevier B.V. All rights reserved.