Quantifying HIV-1-specific CD8 (+) T-cell responses using ELISPOT and cytokine flow cytometry.
Quantifying HIV-1-specific CD8 (+) T-cell responses using ELISPOT and cytokine flow cytometry.
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使用 ELISPOT 和细胞因子流式细胞术定量 HIV-1 特异性 CD8 ( ) T 细胞反应。
DOI：
10.1007/978-1-59745-170-3_24
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Lemongello,Donna
中科院分区：
文献类型：
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作者：
Shacklett,BarbaraL;Critchfield,JWilliam;Lemongello,Donna
自从人类免疫缺陷病毒（HIV-1）引起艾滋病的最初描述和表征以来，许多研究小组已经表征了对这种病毒的免疫反应。许多努力已经指向确定可能对疫苗和免疫疗法的开发有用的保护的潜在相关性。此外，一些研究集中在比较具有快速与缓慢疾病进展特征的患者，以试图确定“成功”免疫应答的特征。虽然我们对宿主-病原体关系的理解仍存在许多差距，但在过去20年中，在阐明对HIV-1的适应性细胞介导反应方面取得了很大进展。近年来，这些研究受益于分析抗原特异性CD 8 + T细胞功能的新方法的发展，特别是ELISPOT测定和细胞因子流式细胞术。本章提供了这两种方法的简单协议。
Since the initial description and characterization of the agent that causes AIDS, human immunodeficiency virus (HIV-1), numerous research groups have characterized immune responses to this virus. Much effort has been directed towards identifying potential correlates of protection that may be useful for the development of vaccines and immunotherapies. In addition, several investigations have focused on comparing patients with rapid vs. slow disease progression profiles in an attempt to identify the characteristics of a “successful” immune response. Although many gaps remain in our understanding of the host–pathogen relationship, great progress has been made during the past 20 years in elucidating the adaptive, cell-mediated response to HIV-1. These investigations have benefited in recent years from the development of new approaches to the analysis of antigen-specific CD8+ T-cell function, notably the ELISPOT assay and cytokine flow cytometry. This chapter provides simple protocols for these two methods.