Circulating leukocyte counts, activation, and degranulation in Dahl hypertensive rats.
Circulating leukocyte counts, activation, and degranulation in Dahl hypertensive rats.
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DOI：
10.1161/01.res.76.2.276
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发表时间：
1995-02
影响因子：
20.1
通讯作者：
Katherine A. Shen;F. DeLano;Benjamin W. Zweifach;G. Schmid-Schönbein
中科院分区：
文献类型：
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作者：
Katherine A. Shen;F. DeLano;Benjamin W. Zweifach;G. Schmid-Schönbein
先前的证据表明，患有自发性高血压的大鼠的循环白细胞平均约为血压正常的大鼠（Wistar-Kyoto大鼠）的两倍。由于如此高水平的白细胞可能会增加高血压动物血管并发症的风险，因此确定其他形式的高血压是否存在类似的紊乱是有用的。本研究涉及循环白细胞的性质，在大鼠表现出另一种形式的实验性高血压;达尔盐敏感（Dahl-S）高血压大鼠与达尔盐抗性（Dahl-R）对照大鼠进行了比较。进行测量以确定以下内容：循环血细胞比容水平、白细胞计数、分类计数、活化白细胞数量（通过硝基蓝四唑[NBT]还原法）、体外白细胞粘附和中性粒细胞CD-18表达、单个中性粒细胞和血浆中的碱性磷酸酶活性以及中性粒细胞中的髓过氧化物酶活性。实验组由Dahl-S和Dahl-R大鼠组成，维持6周的6%NaCl饮食。结果显示，Dahl-S大鼠的总白细胞数量、中性粒细胞和单核细胞计数以及NBT阳性中性粒细胞和单核细胞数量高度显著升高，但Dahl-R大鼠没有。盐处理的Dahl-S大鼠的中性粒细胞中碱性磷酸酶和髓过氧化物酶活性显着丧失，但未处理的Dahl-S或Dahl-R大鼠的中性粒细胞中则没有。中性粒细胞粘附在体外试验条件下没有显着差异，发现这两种菌株保持在盐饮食。（250字处删节）
Previous evidence has shown that rats with spontaneous hypertension have on average about twice as many circulating leukocytes in comparison with their normotensive counterparts, the Wistar-Kyoto rats. Since such high levels of leukocytes may increase the risk for vascular complications for hypertensive animals, it is useful to ascertain whether a comparable derangement is present in other forms of hypertension. The present study deals with the properties of the circulating leukocytes in rats exhibiting another form of experimental hypertension; Dahl salt-sensitive (Dahl-S) hypertensive rats were compared with Dahl salt-resistant (Dahl-R) control rats. Measurements were performed to determine the following: circulating hematocrit levels, leukocyte counts, differential counts, number of activated leukocytes (by means of nitro blue tetrazolium [NBT] reduction), leukocyte adhesion in vitro and neutrophil CD-18 expression, alkaline phosphatase activity in individual neutrophils and in the plasma, and myeloperoxidase activity in neutrophils. The experimental cohort consisted of Dahl-S and Dahl-R rats maintained for a 6-week period on a 6% NaCl diet. The results show a highly significant elevation in the number of total leukocytes, neutrophil and monocyte counts, and NBT-positive neutrophils and monocytes in Dahl-S but not Dahl-R rats. There was a significant loss of alkaline phosphatase and myeloperoxidase activity in the neutrophils of the salt-treated Dahl-S rats but not in the neutrophils of the untreated Dahl-S or Dahl-R rats. No significant differences were found in neutrophil adhesion under in vitro test conditions between the two strains maintained on the salt diet.(ABSTRACT TRUNCATED AT 250 WORDS)