Isolation of candidate genes for apomixis in Poa pratensis L.
Isolation of candidate genes for apomixis in Poa pratensis L.
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DOI：
10.1007/s11103-004-5211-y
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发表时间：
2004-12-01
影响因子：
5.1
通讯作者：
Falcinelli, M
中科院分区：
文献类型：
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作者：
Albertini, E;Marconi, G;Falcinelli, M
无融合生殖的基本特征是胚胎是由无融合生殖产生的胚囊中未减数分裂的卵子自主孤雌生殖形成的。因此，后代的基因构成通常与母本完全相同，这是植物育种者非常感兴趣的一个特征。如果能很好地理解和利用无融合生殖，就可以无限繁殖携带复杂基因的优良杂交种或特定的基因类型。对调节生殖事件的分子机制的深入了解可以为理解无融合生殖途径的遗传控制提供基本的贡献。在草地早熟禾中，通过cDNA-AFLP的mRNA图谱分析方法，我们可以看到总共2248个转录本衍生的片段，并分离出179个在开花时无融合生殖和有性生殖之间存在定性或定量差异的序列，这些序列发生在无融合生殖的初级阶段。选择了三个EST进行进一步的分子鉴定，因为它们的表达模式和BLAST信息检索。通过RACE回收新分离基因的全长，并通过RT-PCR分析它们的时间表达模式。报道并讨论了它们在植物孢子发生、配子发生和胚胎发生过程中所需的细胞信号转导级联和转运事件中的可能作用。
The essential feature of apomixis is that an embryo is formed autonomously by parthenogenesis from an unreduced egg of an embryo sac generated through apomeiosis. The genetic constitution of the offspring is, therefore, usually identical to the maternal parent, a trait of great interest to plant breeders. If apomixis were well understood and harnessed, it could be exploited to indefinitely propagate superior hybrids or specific genotypes bearing complex gene sets. A fundamental contribution to the understanding of the genetic control of the apomictic pathway could be provided by a deep knowledge of molecular mechanisms that regulate the reproductive events. In Poa pratensis the cDNA-AFLP method of mRNA profiling allowed us to visualize a total of 2248 transcript-derived fragments and to isolate 179 sequences that differed qualitatively or quantitatively between apomictic and sexual genotypes at the time of flowering when the primary stages of apomixis occur. Three ESTs were chosen for further molecular characterization because of their cDNA-AFLP expression pattern and BLAST information retrieval. The full-lengths of the newly isolated genes were recovered by RACE and their temporal expression patterns were assessed by RT-PCR. Their putative role in cell signaling transduction cascades and trafficking events required during sporogenesis, gametogenesis and embryogenesis in plants is reported and discussed.