ORAOV1 is amplified in oral squamous cell carcinoma
ORAOV1 is amplified in oral squamous cell carcinoma
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DOI：
10.1111/j.1600-0714.2011.01053.x
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Nunes, Fabio Daumas
中科院分区：
文献类型：
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作者：
Aquino Xavier, Flavia Calo;Rodini, Camila Oliveira;Nunes, Fabio Daumas
背景技术背景：口腔癌过表达基因1（oral cancer overexpressed 1，ORAOV 1）在口腔癌细胞系中的扩增和过表达被认为是11 q13区域的候选癌基因。由于基因扩增通常会导致基因表达水平的增加，我们的目的是通过定量分析来验证口腔鳞状细胞癌（OSCC）中ORAOV 1基因状态和mRNA表达之间的关系，并与患者的临床和病理特征相关联。通过qPCR和RT-qPCR评估OSCC细胞系以及肿瘤和非肿瘤组织中的ORAOV 1扩增和表达水平。33例OSCC患者的肿瘤手术切缘。结果：3株11 q13扩增的口腔鳞癌细胞株中，与未扩增的细胞株相比，ORAOV 1基因表达水平和拷贝数均明显增高（单因素方差分析，P < 0.05）。在肿瘤样本中，ORAOV 1 mRNA水平和DNA拷贝数之间存在弱相关性（斯皮尔曼，P = 0.07）。虽然ORAOV 1在肿瘤中扩增（Wilcoxon，P < 0.01），但与肿瘤相比，边缘的高水平转录本没有显示差异（Wilcoxon，P = 0.85）。侵袭性和生存率没有表现出统计学差异，这两个事件在OSCC.CONCLUSION：在非肿瘤边缘样本中的ORAOV 1的过度表达，可以发生在扩增的情况下。在OSSC中，ORAOV 1扩增和表达之间的弱相关性表明，ORAOV 1表达可以通过基因扩增以外的机制来调节。口腔病理学医学杂志（2012）41：5460
BACKGROUND: Oral cancer overexpressed 1 (ORAOV1) was found as a candidate oncogene in the 11q13 chromosomal region, based on its amplification and overexpression in oral cancer cell lines. Because gene amplification often leads to increased levels of gene expression, we aimed to verify the relationship between ORAOV1 gene status and mRNA expression primarily in oral squamous cell carcinoma (OSCC) by quantitative assay, correlating with clinical and pathological characteristics in patients.METHODS: Levels of ORAOV1 amplification and expression were evaluated by qPCR and RT-qPCR in OSCC cell lines and in tumor and non-tumoral surgical margins from 33 patients with OSCC. All subjects were smokers and habitual alcohol drinkers, mostly men above 40 years of age and with a single primary tumor.RESULTS: ORAOV1 exhibited increased gene expression levels as well as higher copy number in three OSCC cell lines with 11q13 amplified chromosomal region when compared with the OSCC cell line without the amplification (one-way ANOVA, P < 0.05). Weak correlation between ORAOV1 mRNA levels and DNA copy number was seen in tumor samples (Spearman, P = 0.07). Although ORAOV1 was amplified in tumor (Wilcoxon, P < 0.01), high levels of transcripts in margin did not reveal differences in comparison with tumor (Wilcoxon, P = 0.85). Aggressiveness and survival rate did not demonstrate statistical difference for both events in OSCC.CONCLUSION: The overexpression of ORAOV1 in non-tumoral margin samples can occur in the absence of amplification. The weak correlation between ORAOV1 amplification and expression in OSSC suggests that ORAOV1 expression can be regulated by mechanisms other than gene amplification. J Oral Pathol Med (2012) 41: 5460