Cortical and subcortical patterns of synaptophysinlike immunoreactivity in Alzheimer's disease.
Cortical and subcortical patterns of synaptophysinlike immunoreactivity in Alzheimer's disease.
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发表时间：
1991
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通讯作者：
E. Masliah;R. Terry;Michael;Alford;R. DeTeresa;L. Hansen
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作者：
E. Masliah;R. Terry;Michael;Alford;R. DeTeresa;L. Hansen
突触素样免疫反应的定量是研究正常和受损神经系统突触前末梢的一种有价值的方法。本报告表明，在控制大脑中，突触免疫染色的主要模式在新皮层是一个均匀分布的密集颗粒免疫标记的神经元，而在paleocortex和皮层下的大脑区域的突触前末梢分布沿着树突状arborizations或周围的神经元胞体。阿尔茨海默病组织的免疫化学和免疫组织化学分析表明，额叶和顶叶皮质提出了最严重和广泛的损失，与45%的损失突触素免疫反应。这些区域显示出平均35%的大神经元损失。视觉皮质、海马、内嗅皮质、Meynert基底核和蓝斑显示出一定程度的丢失，但程度较小。除了这种损失，突触前末梢的基本组织模式也发生了改变，出现了大量的、扩大的突触素标记的末梢。这项研究进一步支持了突触病理学在阿尔茨海默病中的作用。
Quantification of synaptophysinlike immunoreactivity is a valuable method for studying the presynaptic terminals in the normal and damaged nervous system. The present report shows that in the control brain, the predominant pattern of synaptic immunostaining in the neocortex was that of an evenly distributed densely granular immunolabeling of the neuropil, while in the paleocortex and in subcortical areas of the brain most of the presynaptic terminals were distributed along the dendritic arborizations or around the neuronal somata. The immunochemical and the immunohistochemical analysis of the Alzheimer's disease tissue showed that the frontal and parietal cortex presented the most severe and widespread loss, with a 45% loss in synaptophysin immunoreactivity. These areas showed an average 35% loss of large neurons. The visual cortex, hippocampus, entorhinal cortex, nucleus basalis of Meynert, and locus ceruleus displayed some degree of loss, but to a lesser extent. In addition to this loss, the basic patterns of organization of the presynaptic terminals were altered, with the presence of abundant, enlarged synaptophysin-labeled terminals. This study further supports the role of synaptic pathology in Alzheimer's disease.