NEURITE EXTENSION BY PERIPHERAL AND CENTRAL NERVOUS-SYSTEM NEURONS IN RESPONSE TO SUBSTRATUM-BOUND FIBRONECTIN AND LAMININ
NEURITE EXTENSION BY PERIPHERAL AND CENTRAL NERVOUS-SYSTEM NEURONS IN RESPONSE TO SUBSTRATUM-BOUND FIBRONECTIN AND LAMININ
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DOI：
10.1016/0012-1606(83)90350-0
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
FURCHT, LT
中科院分区：
文献类型：
--
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
分离的神经元从胚胎鸡背根和交感神经节（外周神经元）和从脊髓和视网膜（中枢神经系统神经元）培养塑料基板处理纯化的纤连蛋白和层粘连蛋白。中枢神经元和外周神经元都附着在层粘连蛋白上，并在层粘连蛋白上延伸神经突。只有外周神经元在纤连蛋白上启动神经突;在相同条件下培养的中枢神经元聚集成簇，并且不延伸神经突。神经突的长度，神经突的数量开始和纤维连接蛋白和层粘连蛋白处理的基质上的神经突分支的程度进行了评价，并与类似的测量聚-L-赖氨酸处理的塑料神经元的反应进行了比较。多聚赖氨酸为神经突的伸长提供了一个粘附表面，但纤连蛋白和层粘连蛋白似乎能促进更快的神经突伸长。显然，神经元与这些糖蛋白的相互作用可能涉及神经元特异性细胞表面成分。这些反应层粘连蛋白和纤连蛋白在体外可能与这些糖蛋白在特定的细胞外环境中的存在或不存在，在特定的发育阶段。
Dissociated neurons from embryonic chick dorsal root and sympathetic ganglia (peripheral neurons) and from spinal cord and retina (central nervous system neurons) were cultured on plastic substrata treated with purified fibronectin and laminin. Both central and peripheral neurons attached to and extended neurites on laminin. Only peripheral neurons initiated neurites on fibronectin; central neurons cultured under identical conditions aggregated into clusters and did not extend neurites. Neurite length, number of neurites initiated and extent of neurite branching on fibronectin- and laminin-treated substrata were evaluated and compared with similar measurements of neuronal response to poly-L-lysine-treated plastics. Poly-L-lysine provides an adhesive surface for neurite elongation, but fibronectin and laminin appear to promote more rapid neurite elongation. Evidently, neuronal interaction with these glycoproteins may involve neuron-specific cell surface components. These responses to laminin and fibronectin in vitro may be related to the presence or absence of these glycoproteins in specific extracellular environments during specific developmental stages.