Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.
Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.
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DOI：
10.1091/mbc.e20-12-0807
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发表时间：
2021-08-15
影响因子：
3.3
通讯作者：
Gould KL
中科院分区：
文献类型：
--
作者：
Mangione MC;Chen JS;Gould KL
遗传物质和细胞质的分裂在空间和时间上协调以确保基因组的完整性。这种协调部分由主要的细胞周期调节因子细胞周期蛋白依赖性激酶（Cdk 1）介导。Cdk 1活性在有丝分裂期间达到峰值，但在有丝分裂退出/胞质分裂期间Cdk 1活性降低，并且其底物的磷酸化被包括Cdc 14、PP 1、PP 2A和PP 2B的各种磷酸酶逆转。已知Cdk 1磷酸化肌动蛋白和肌球蛋白为基础的细胞动力学环（CR），介导酵母和动物细胞的分裂的几个组件。在这里，我们表明，Cdk 1也磷酸化裂殖酵母的裂殖酵母CR组件桩蛋白Pxl 1。我们确定，Cdc 14磷酸酶Clp 1和PP 1磷酸酶Dis 2有助于Pxl 1去磷酸化在有丝分裂出口，但PP 2B/钙调磷酸酶不。通过Cdk 1阻止Pxl 1磷酸化导致Pxl 1水平增加，Pxl 1过早募集到分裂位点，以及CR收缩持续时间增加。在体外Cdk 1介导的Pxl 1磷酸化抑制其与细胞动力学支架Cdc 15的F-BAR结构域的相互作用，从而破坏Pxl 1募集的主要机制。因此，Pxl 1是S.粟酒酵母Cdk 1和相反的磷酸酶协调有丝分裂和胞质分裂。
Divisions of the genetic material and cytoplasm are coordinated spatially and temporally to ensure genome integrity. This coordination is mediated in part by the major cell cycle regulator cyclin-dependent kinase (Cdk1). Cdk1 activity peaks during mitosis, but during mitotic exit/cytokinesis Cdk1 activity is reduced, and phosphorylation of its substrates is reversed by various phosphatases including Cdc14, PP1, PP2A, and PP2B. Cdk1 is known to phosphorylate several components of the actin- and myosin-based cytokinetic ring (CR) that mediates division of yeast and animal cells. Here we show that Cdk1 also phosphorylates the Schizosaccharomyces pombe CR component paxillin Pxl1. We determined that both the Cdc14 phosphatase Clp1 and the PP1 phosphatase Dis2 contribute to Pxl1 dephosphorylation at mitotic exit, but PP2B/calcineurin does not. Preventing Pxl1 phosphorylation by Cdk1 results in increased Pxl1 levels, precocious Pxl1 recruitment to the division site, and increased duration of CR constriction. In vitro Cdk1-mediated phosphorylation of Pxl1 inhibits its interaction with the F-BAR domain of the cytokinetic scaffold Cdc15, thereby disrupting a major mechanism of Pxl1 recruitment. Thus, Pxl1 is a novel substrate through which S. pombe Cdk1 and opposing phosphatases coordinate mitosis and cytokinesis.
影响因子：
2.6
作者：
Chen, Jun-Song;Beckley, Janel R.;Ren, Liping;Feoktistova, Anna;Jensen, Michael A.;Rhind, Nicholas;Gould, Kathleen L.
通讯作者：
Gould, Kathleen L.