RAPD and seed coat morphology variation in annual and perennial species of the genus Cicer L.
RAPD and seed coat morphology variation in annual and perennial species of the genus Cicer L.
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Cicer L 属一年生和多年生物种的 RAPD 和种皮形态变化。
DOI：
10.1023/b:gres.0000034584.43689.f1
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发表时间：
2004
影响因子：
2
通讯作者：
H. Yamaguchi
中科院分区：
文献类型：
--
作者：
F. Javadi;H. Yamaguchi
本文对鹰嘴豆、鹰嘴豆根据RAPD和S.E.M.种皮特征使用三个外群分类群（透镜ervoides，山豌豆和豌豆）。在66个十聚体随机引物中，有8个引物扩增出87个信息片段。利用Jaccard相似性系数对多态性片段进行相邻聚类分析，结果表明C. arietinums和Cicer属中有两个主要的类群，第一个类群包括四个单角组（Sect.Monocicer）种：Cicer. echinospermum、刺孢C. arietinum，C. reticulatum和伊朗C.双轭。第二个集群包含属于Chamaecicer、Polycicer和Acanthocicer组的一年生和多年生物种。RAPD表型图上形态和生态性状的特征状态表明不存在单系切割。研究结果表明，一年生和多年生物种间的遗传差异可能是单冰器组物种快速形成的结果。有必要重新考虑传统的多环芳烃和多环芳烃的分类。C.在DNA水平上，不能将10个山羊草属的植物进行单独的分类，且山羊草属的遗传变异水平较低。白羊座可能是驯化过程中的瓶颈。
Molecular and morphological variation of six perennial and five annual species including domesticated chickpea,C. arietinum, were inferred on the basis of RAPD and S.E.M. seed coat features using three outgroup taxa (Lens ervoides,Lathyrus japonicaandPisum sativum). Of the 66 decamer arbitrary primers tested, eight primers revealed 87 informative fragments. Neighbor-joining cluster analysis using Jaccard's coefficient of similarity on the basis of polymorphic fragments indicated a narrow variation inC. arietinumand recognized two major clusters in the genusCicer.The first one included four species of sect. Monocicer:C. echinospermum,C. arietinum,C. reticulatumand Iranian material ofC. bijugum. The second cluster contained annual and perennial species belonging to sections Chamaecicer, Polycicer and Acanthocicer. The character state of morphological and ecological traits on the RAPD phenogram indicated no monophyletic incision. Our results suggested that the high genetic difference between annual and perennial species might be regarded as a rapid speciation of section Monocicer. Reconsideration of traditional classification of sections Polycicer and Acanthocicer is necessary. The ‘Desi’ and ‘Kabuli’ types ofC. arietinumcould not be separately grouped at the DNA level, and the low level of genetic variation ofC. arietinummay result from a bottleneck during the domestication process.