Nutritional study on Embellisia astragali, a fungal pathogen of milk vetch (Astragalus adsurgens)
Nutritional study on Embellisia astragali, a fungal pathogen of milk vetch (Astragalus adsurgens)
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DOI：
10.1007/s10482-009-9310-2
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Y. Li;Z. Nan
中科院分区：
文献类型：
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作者：
Y. Li;Z. Nan
黄芪润孢菌是一种在黄芪（沙剂黄芪）中产生的强毒病原体。为了确定真菌的营养需求，在25℃的黑暗条件下，在9个碳源、9个氮源和13种生长培养基上培养真菌。4周和12周后测定生长速率和产孢量。所有碳源都支持生长，但只有可溶性淀粉、菊粉和葡萄糖支持孢子形成。总的来说，双糖和多糖比单糖生长得更好。与NH4+-N和尿素相比，真菌在所有NO3−-N、氨基n和其他有机n（如蛋白胨）上生长很少。以葡萄糖或可溶性淀粉为唯一碳源的氮源几乎都不产孢，分生孢子非常稀疏。在大多数半确定培养基上比在确定培养基上生长和产孢更好，这表明植物或动物材料中的某些成分可能对真菌至关重要。氮源试验第12周、培养基试验第4周和第12周产孢量与生长率呈正相关。该菌适宜在琼脂内生长，生长速率小于1.18 mm day−1。
Embellisia astragaliis a strong, virulent pathogen that develops within milk vetch (Astragalus adsurgens). In order to determine nutrient requirements, the fungus was cultured on 9 carbon sources, 9 nitrogen sources, and 13 growth media in the dark at 25°C. Growth rates and sporulation capacity were measured after 4 and 12 weeks. All carbon sources supported growth, but only soluble starch, inulin, and dextrose supported sporulation. In general, better growth was obtained on disaccharides and polysaccharides than on monosaccharides. Compared with no growth on NH4+-N and urea, the fungus grew little on all NO3−-N, amino-N, and other organic-N such as peptone. There was no sporulation or very sparse conidia on almost all nitrogen sources with supplied dextrose or soluble starch as sole carbon source. The better growth and sporulation on most of the semidefined media than on defined media indicates that some components in plant or animal material may be vital to the fungus. Sporulation was positively correlated with growth rate in N source experiment at 12 weeks and in growth media experiment at 4 and 12 weeks. The fungus favors grow within agar with growth rate less than 1.18 mm day−1.