Involvement of changes in stratum corneum keratin in wrinkle formation by chronic ultraviolet irradiation in hairless mice
Involvement of changes in stratum corneum keratin in wrinkle formation by chronic ultraviolet irradiation in hairless mice
复制标题
DOI：
10.1034/j.1600-0625.12.s2.4.x
复制
发表时间：
2003-01-01
影响因子：
3.6
通讯作者：
Ichihashi, M
中科院分区：
文献类型：
--
作者：
Kambayashi, H;Odake, Y;Ichihashi, M
长期暴露于紫外线（UV）辐射会改变皮肤的成分。我们以前报道，慢性低剂量紫外线照射诱导皱纹的形成，但没有显着改变无毛小鼠的真皮成分。在本研究中，我们研究了紫外线照射引起的表皮变化是否有助于皱纹的形成。用UVB（20 mJ/cm（2））和UVA（14 J/cm（2））的最小红斑剂量（MED）的三分之一照射无毛小鼠10周，并在不照射的情况下随访24周。在照射后检测到细小皱纹，即使在停止照射后24周也存在。在总膨胀的粘弹性比例（UV/Ue）的增加被确认在照射小鼠，这可能与皱纹的形成。在表皮中，在紫外线照射10周后观察到经表皮水分损失量增加和角质层含水量减少，在24周的观察期内均未恢复。此外，有一个显着增加角质层角蛋白的量后，10周的照射，并持续在24周的随访。我们的研究结果表明，慢性低剂量紫外线照射主要改变表皮，而不是皮肤的真皮成分。此外，我们的研究结果表明，角质层角蛋白含量的增加可能涉及皮肤的物理性质的改变。这个过程可能是皱纹形成的早期事件之一。
Chronic exposure to ultraviolet (UV) radiation alters components of the skin. We previously reported that chronic low-dose UV irradiation induced wrinkle formation but did not significantly alter dermal components of hairless mice. In the present study, we examined whether the epidermal changes by UV irradiation could contribute to wrinkle formation. Hairless mice were irradiated with one-third the minimum erythema dose (MED) of UVB (20 mJ/cm(2)) and UVA (14 J/cm(2)) for 10 weeks, and followed up for another 24 weeks without irradiation. Fine wrinkles were detected following irradiation, which existed even 24 weeks after cessation of irradiation. An increase in the viscoelastic proportion of the total distension (Uv/Ue) was recognized in irradiated mice, which may be related to wrinkle formation. In the epidermis, an increase in the amount of transepidermal water loss and a decrease in the water content of the stratum corneum were seen after 10 weeks of UV irradiation, neither of which recovered during the 24-week observation period. In addition, there was a significant increase in the amount of stratum corneum keratin after 10 weeks of irradiation, which persisted during the 24-week follow-up. The results of our study suggest that chronic low-dose UV irradiation primarily alters the epidermal,, rather than the dermal components of the skin. In addition, our results indicate that the increased keratin content of the stratum corneum may be involved in the alteration of the physical properties of the skin. This process could be one of the early events of wrinkle formation.