Bone Augmentation Experiment using Coral on the Skull of Rat
Bone Augmentation Experiment using Coral on the Skull of Rat
复制标题
大鼠头骨上珊瑚的骨增量实验
DOI：
10.11344/nano.5.109
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
A. Tanaka
中科院分区：
文献类型：
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作者：
T. Nishikawa;Tomoharu Okamura;Takanao Ono;H. Matsushita;K. Imai;Y. Honda;M. Hidaka;N. Matsumoto;S. Takeda;A. Tanaka
骨量增长（骨增量）是理想的，以改善咬合功能的颌骨骨量减少。因此，需要具有足够机械强度的支架来确保用于新骨形成的空间。由于珊瑚是一种具有机械强度的多孔支架，我们尝试了一种新的骨增量方法，使用它作为支架。材料与方法：将珊瑚片植入大鼠颅骨表面骨膜下。植入手术后12个月，给予钙黄绿素和茜素红，3天后取出具有植入部分的颅骨，并用光学显微镜和共聚焦激光扫描显微镜（CLSM）观察标本。结果如下：术后12个月，植入的珊瑚盘仍然存在，同时观察到骨增量以及新骨形成，包括珊瑚盘内腔中的许多骨细胞。该区域炎性细胞和异物巨细胞较少。这些发现表明，尽管珊瑚盘的生物可吸收性较弱，但它是一种有前途的颅骨骨增量支架材料。
Bone mass growth (bone augmentation) is desirable to improve occlusal function in jaws with a decrease in bone mass. Therefore, scaffold with sufficient mechanical strength to secure space for new bone formation is needed. Since coral is a porous scaffold with mechanical strength, we tried a new method for bone augmentation using it as the scaffold. Materials and Methods: Coral disks were implanted under periosteum on the surface of rat skull. Twelve months after the implant procedure, calcein and alizarin red were administered and three days later the skull with the implanted portion was removed and the specimen was observed with a light microscope and confocal laser scanning microscope (CLSM). Results: At twelve months postoperatively, the implanted coral disks remained, while the bone augmentation was observed together with new bone formation including many osteocytes in the inner cavities of the coral disk. There were few inflammatory cells and foreign body giant cells in this area. These findings suggest that though coral disk is weakly bioabsorbable, it is a promising scaffolding material for bone augmentation on the skull.