The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation.
The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation.
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新洋葱伯克霍尔德菌 H111 中的 Bep 基因簇编码生物膜形成所必需的水不溶性胞外多糖。
DOI：
10.1016/j.carbpol.2022.120318
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发表时间：
2023
影响因子：
11.2
通讯作者：
Cescutti,Paola
中科院分区：
文献类型：
--
作者：
Bellich,Barbara;Terán,LucreciaC;Fazli,MagnusM;Berti,Francesco;Rizzo,Roberto;Tolker-Nielsen,Tim;Cescutti,Paola
新洋葱伯克霍尔德氏菌是一种从囊性纤维化患者中分离出来的机会致病菌，它会导致感染，这些感染很难用抗生素治疗，有时会导致致命的后果。生物被膜是B. cenocepacia，并与感染的持续性和增加的耐药性抗生素。在生物膜中，胞外多糖具有重要作用，赋予机械稳定性和抗生素耐受性。从B. cenocepaciaH 111生物膜：富含鼠李糖并含有L-Man残基的水溶性多糖，以及由葡萄糖、半乳糖和甘露糖制成的水不溶性聚合物。在本工作中，由B. cenocepaciaH 111 bepA-L基因簇被鉴定为水不溶性胞外多糖，使用突变菌株和纯化多糖的NMR光谱。同时也证明了B. cenocepaciaH 111野生型菌株在薄膜中产生水不溶性外泌多糖，因此强调了其在体内感染中的潜在重要性。
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait ofB. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated fromB. cenocepaciaH111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded byB. cenocepaciaH111bepA-Lgene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that theB. cenocepaciaH111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.