LABOR AND INFECTION .2. BACTERIAL-ENDOTOXIN IN AMNIOTIC-FLUID AND ITS RELATIONSHIP TO THE ONSET OF PRETERM LABOR
LABOR AND INFECTION .2. BACTERIAL-ENDOTOXIN IN AMNIOTIC-FLUID AND ITS RELATIONSHIP TO THE ONSET OF PRETERM LABOR
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DOI：
10.1016/0002-9378(88)90216-5
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发表时间：
1988-05-01
影响因子：
9.8
通讯作者：
HOBBINS, JC
中科院分区：
文献类型：
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作者：
ROMERO, R;ROSLANSKY, P;HOBBINS, JC
我们以前曾报道过革兰氏阴性羊膜内感染患者羊水中内毒素的检测。内毒素或脂多糖是一种有效的生物制品，能够诱导前列腺素从几种细胞类型中释放，因此，在羊膜内感染的情况下，可能参与人类分娩的发生。本文介绍了一种定量羊水中内毒素的技术。该方法采用计算机辅助定量鲎试剂和内毒素之间的浊度反应。制备培养阴性羊水中大肠杆菌内毒素的系列稀释液，并在测定中运行样品。发现羊水可增强反应，并且需要1：20的稀释度使该生物流体的行为类似于无热原水。动态浊度法检测羊水内毒素的灵敏度为40 pg/ml。应用此方法对26例羊水内毒素感染合并胎膜早破患者的羊水内毒素浓度进行了测定。产程活跃患者的内毒素浓度（中位数= 47，514 pg/ml）高于非产程患者（中位数= 635 pg/ml）（p < 0.025）。因此，早产妇女羊水中内毒素的中位数浓度高于未分娩的患者。
We have previously reported the detection of endotoxin in the amniotic fluid of patients with gram-negative intraamniotic infection. Endotoxin or lipopolysaccharide is a potent biologic product capable of inducing prostaglandin release from several cell types and, therefore, may be involved in the onset of human parturition in the presence of intraamniotic infection. This article describes a technique for the quantification of endotoxin in amniotic fluid. The method uses a computer-assisted quantification of the turbidimetric reaction between the Limulus amebocyte lysate and endotoxin. Serial dilutions of Escherichia coli endotoxin in culture-negative amniotic fluid were prepared, and the samples were run in the assay. Amniotic fluid was found to enhance the reaction, and a dilution of 1:20 was required for this biologic fluid to behave similarly to pyrogen-free water. The sensitivity of this kinetic turbidimetric technique in the detection of endotoxin in amniotic fluid was 40 pg/ml. This method was applied to the quantification of endotoxin concentration in amniotic fluid in 26 patients with intraamniotic infection and premature rupture of membranes. Patients in active labor had higher concentrations of endotoxin (median = 47,514 pg/ml) than nonlaboring patients (median = 635 pg/ml) (p < 0.025). Therefore, women with preterm labor had a higher median concentration of endotoxin in amniotic fluid than patients who were not in labor.