FEASIBILITY OF RATING FIRE BLIGHT SUSCEPTIBILITY OF PEAR CULTIVARS (PYRUS-COMMUNIS) ON INVITRO MICROCUTTINGS
FEASIBILITY OF RATING FIRE BLIGHT SUSCEPTIBILITY OF PEAR CULTIVARS (PYRUS-COMMUNIS) ON INVITRO MICROCUTTINGS
复制标题
DOI：
10.1051/agro:19880806
复制
发表时间：
1988-01-01
期刊：
影响因子：
--
通讯作者：
DURON, M
中科院分区：
文献类型：
--
作者：
BRISSET, MN;PAULIN, JP;DURON, M
火疫病的典型症状（软泥产生和坏死）可以通过宿主植物的体外微插条的接种轻松获得。该研究的目的是确定是否可以在体外评估梨品种对火疫病的敏感性。在一项基于体外繁殖的几个梨属品种（敏感和抗性）的研究中，分析了不同外部因素（接种方法、接种浓度、生长条件）对症状定量和定性表达的影响。在果园中已经注意到的结果的可变性在这种植物材料上并没有减少。体外微切割的接种通常会高估敏感性。然而，有时观察到敏感品种的感受性下降。因此，体外微切割试验似乎不适合根据不同的敏感性水平来准确区分品种。然而，这些测试可能有助于从群体中筛选出具有高水平耐药性的个体（即在诱变治疗或诱导体细胞克隆变异之后）。
Typical symptoms of fire blight (ooze production and necrosis) can easily be obtained from the inoculation of in vitro microcuttings of a host plant. The purpose of the study was to determine whether the susceptibility of pear cultivars to fire blight can be evaluated in vitro. In a work based on several Pyrus cultivars (susceptible and resistant) propagated in vitro, the effects of different external factors (method of inoculation, concentration of inoculum, growing conditions) on quantitative and qualitative expression of symptoms were analyzed. The variability of the results, already noticed in orchards, was not lessened on this plant material. Inoculations on in vitro microcuttings usually overestimated susceptibility. However, a decrease of the receptivity of susceptible cultivars was sometimes observed. Therefore tests on in vitro microcuttings do not seem to be suitable for accurate discrimination between cultivars based on their different levels of susceptibility. Nevertheless these tests may be useful for selecting out individuals with high levels of resistance from a population (i.e. after mutagenic treatment or induction of somaclonal variations).