DETERMINATION OF LANDIOLOL IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
DETERMINATION OF LANDIOLOL IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
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DOI：
10.1080/10826076.2011.591022
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发表时间：
2011-01-01
影响因子：
1.3
通讯作者：
Yamamoto, Koujirou
中科院分区：
文献类型：
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作者：
Aomori, Tohru;Matsumoto, Naoki;Yamamoto, Koujirou
兰地洛尔是一种超短效β（1）-肾上腺素能受体阻滞剂，用于有心脏事件风险的围手术期患者。该药物在血液中被假胆碱酯酶非常迅速地水解。以毒扁豆碱（终浓度为10（-4）mol/L）为胆碱酯酶抑制剂，建立了一种简便、快速测定兰地洛尔的高效液相色谱法。在C-18柱上用20 mM KH 2 PO 4/乙腈（70/30，v/v）实现色谱分离。普萘洛尔用作内标。使用荧光检测器（激发220 nm和发射310 nm）检测峰。兰地洛尔和普萘洛尔的保留时间分别为5.6 min和6.7 min。定量下限估计为0.1 μ g/mL。批间和批内精密度在1.8%~ 12.6%之间变化，准确度在-8.1%~7.7%之间变化。本法可用于人体静脉注射兰地洛尔后血药浓度的测定。
Landiolol is an ultra-short-acting beta(1)-adrenergic receptor-blocking agent that is used with perioperative patients who are at risk of cardiac events. The drug is hydrolyzed very rapidly by pseudocholinesterase in the blood. Using physostigmine (final concentration: 10(-4) mol/L) as a cholinesterase inhibitor, we developed a simple and rapid quantification method for landiolol using high-performance liquid chromatography. Chromatographic separations were achieved on a C-18 column with 20 mM KH2PO4/acetonitrile (70/30, v/v). Propranolol was used as an internal standard. The peaks were detected using a fluorescence detector (excitation 220 nm and emission 310 nm). The retention times of landiolol and propranolol were 5.6 min and 6.7 min, respectively. The lower limit of quantification was estimated as being 0.1 mu g/mL. The precision of the inter-and intra-assay varied between 1.8% and 12.6%, and the accuracy varied between -8.1% and 7.7%. This method was useful for the determination of landiolol in human plasma after intravenous administration.