Cryptosporidium infection in dogs in Osaka, Japan
Cryptosporidium infection in dogs in Osaka, Japan
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DOI：
10.1016/s0304-4017(02)00204-2
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发表时间：
2002-09-26
影响因子：
2.6
通讯作者：
Iseki, M
中科院分区：
文献类型：
--
作者：
Abe, N;Sawano, Y;Iseki, M
微小隐孢子虫是一种人畜共患病原体，由遗传上不同但形态上相同的基因型组成。最近的分子研究表明，狗可能会传播牛的基因型，这是已知的人类致病。虽然在几个国家进行了犬隐孢子虫感染的大规模研究，但由于缺乏分子分析方法，因此无法准确鉴定分离株。犬与人密切接触，因此，对犬隐孢子虫病的病原学鉴定是控制隐孢子虫病的重要措施。本研究的目的是计算日本大坂市犬隐孢子虫感染的患病率，并对分离株进行分子特征分析。经PCR检测，患病率为9.3%（13/140）。根据诊断片段的测序，发现所有分离株均为犬隐孢子虫（以前称为犬基因型），认为其对人类无致病性。这些结果表明，PCR为基础的诊断方法是一个有用的工具，隐孢子虫感染的诊断和分子流行病学的狗，生活在大坂的狗是不是一个重要的水库人类隐孢子虫病。目前还不清楚为什么C。犬在狗中占优势。需要进一步的研究来了解这种部分寄生。(C)2002 Elsevier Science B. V.保留所有权利。
Cryptosporidium parvum is a zoonotic pathogen composed of genetically distinct but morphologically identical genotypes. Recent molecular study indicates that dogs may transmit the cattle genotype, which is known to be pathogenic to humans. Although large-scale studies of Cryptosporidium infection in dogs have been performed in several countries, the isolates were not accurately identified because of the lack of a method for molecular analysis. It is important to identify the isolates harbored in dogs, which come in close contact with humans, in order to control human cryptosporidiosis. The aim of the present study was to calculate the prevalence of Cryptosporidium infection in dogs in Osaka city, Japan, and to characterize the isolates molecularly. The prevalence was determined to be 9.3% (13/140) by PCR. All isolates were found to be Cryptosporidium canis (previously known as the dog genotype), which is thought to be non-pathogenic in humans, based on the sequencing of diagnostic fragments. These results indicate that PCR-based diagnostic methods are a useful tool for the diagnosis and molecular epidemiology of Cryptosporidium infection in dogs, and that dogs living in Osaka are not a significant reservoir for human cryptosporidiosis. It is unclear why C. canis is dominant in dogs. Further study is required to understand this partial parasitism. (C) 2002 Elsevier Science B.V. All rights reserved.