Relationship of cigarette smoking to the subgingival microbiota
Relationship of cigarette smoking to the subgingival microbiota
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DOI：
10.1034/j.1600-051x.2001.028005377.x
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发表时间：
2001-05-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
--
作者：
Haffajee, AD;Socransky, SS
背景：吸烟与牙龈下微生物群组成的关系尚不清楚。一些研究表明，吸烟者体内某些种类的微生物含量较高，而其他研究未能发现不同吸烟史受试者之间微生物群的差异。因此，本研究的目的是检查当前、过去或从不吸烟的成人受试者中牙龈下物种的患病率、比例和水平。方法：选取年龄在20 ~ 86岁之间、至少20颗牙齿的272名成年受试者进行研究。通过问卷调查获得吸烟史。在基线访视时对除第三磨牙外的所有牙齿每颗牙齿的6个部位进行临床测量。龈下菌斑样本；采用棋盘DNA-DNA杂交的方法，从每位受试者除第三磨牙外的所有牙齿的近中表面分别采集29种龈下菌种。受试者根据吸烟史分为从不吸烟者（n = 124）、过去吸烟者（n = 98）和目前吸烟者（n = 50）。使用单变量和多变量分析来寻找吸烟类别与牙龈下菌种的数量、比例和患病率之间的关系。结果：3个吸烟组中试验种的流行率（定殖点百分比）的差异大于总计数或总计数比例的差异。橘黄色和红色复合体的成员，包括结瘤芽孢杆菌、核仁芽孢杆菌、中间芽孢杆菌、微小芽孢杆菌、黑孢杆菌、连翘芽孢杆菌、牙龈芽孢杆菌和齿状芽孢杆菌，在当前吸烟者中显著高于其他两组。吸烟者和非吸烟者之间患病率的差异是由于在口袋深度的地方有更多的殖民化
Background: The relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. Some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. Thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.Method: 272 adult subjects ranging in age from 20-86 years with at least 20 teeth were recruited for study. Smoking history was obtained using a questionnaire. Clinical measures were taken at 6 sites per tooth at all teeth excluding third molars at a baseline visit. Subgingival plaque samples tr;ere taken from the mesial surface of all teeth excluding third molars in each subject at baseline and assayed individually for counts of 29 subgingival species using checkerboard DNA-DNA hybridization. Subjects were subset according to smoking history into never (n = 124), past (n = 98) and current smokers (n = 50). Uni-variate and multi-variate analyses were used to seek associations between smoking category and the counts, proportions and prevalence of subgingival species.Results: Greater differences were observed for the prevalence (% of sites colonized) of the test species in the 3 smoking groups than were observed for counts or proportions of total counts. Members of the orange and red complexes including E. nodatum, F. nucleatum ss vincentii, P. intermedia, P. micros, P. nigrescens, B. forsythus, P. gingivalis and T. denticola were significantly more prevalent in current smokers than in the other 2 groups. The difference in prevalence between smokers and non-smokers was due to greater colonization at sites with pocket depth