SELECTIVITY OF FLUORESCENT LIPID ANALOGS FOR LIPID DOMAINS
SELECTIVITY OF FLUORESCENT LIPID ANALOGS FOR LIPID DOMAINS
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DOI：
10.1021/bi00567a039
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
WOLF, DE
中科院分区：
文献类型：
--
作者：
KLAUSNER, RD;WOLF, DE
研究了掺入二饱和卵磷脂 (PC) 囊泡中的偶数链长 (n = 10-22) 二酰基-3,3''-吲哚羰花青碘化物 (CndiI) 的相分配偏好。使用两个参数来确定这种相偏好：由染料引起的相变温度 (Tm) 的移动方向，以及由于优先分配到流体中的那些染料在凝胶相中聚集而导致的荧光自猝灭。降低Tm的染料优先分配到液相中；那些提高 Tm 的物质优先分配到凝胶中。根据二肉豆蔻酰基-PC [DMPC]中的这些标准，C10 diI和C12 diI优先分配到流体相中，C14 diI和C16 diI没有表现出优先分配，C18 diI优先分配到凝胶中，C20 diI和C22 diI优先分配到流体中。在二棕榈酰-PC 中，优先模式与在 DMPC 中观察到的相同，只是向更长链长的 diI' 偏移了 2 C。凝胶相多层中这些染料的荧光光漂白恢复的扩散测量显示它们都是固定的，D < 10-10 cm/2/s，而在流体相多层中，它们的扩散系数均为 D .apprx。 10-8 cm2/s，与链条长度无关。在混合相多层膜中，每个 Cn diI 都显示出流动分数，这反映了其相分配偏好。
The phase partition preferences of the even chain length (n = 10-22) diacyl-3,3''-indocarbocyanine iodides (CndiI) incorporated in disaturated lecithin (PC) vesicles were examined. Two parameters were used to determine this phase preference: the direction of shift of the phase transition temperature (Tm) induced by the dyes and the self-quenching of fluorescence due to aggregation in the gel phase of those dyes which preferentially partition into the fluid. Dyes that lower Tm preferentially partition into the fluid phase; those that raise Tm preferentially partition into the gel. By these criteria in dimyristoyl-PC [DMPC], C10 diI and C12 diI preferentially partition into the fluid phase, C14 diI and C16 diI show no preferential partition, C18 diI preferentially partitions into the gel and C20 diI and C22 diI preferentially partition into the fluid. In dipalmitoyl-PC the pattern of preference is identical with that observed in DMPC, only shifted to longer chain length diI''s by 2 C. Diffusion measurements by fluorescence photobleaching recovery of these dyes in gel-phase multilayers showed them all to be immobile, D < 10-10 cm/2/s, while in fluid-phase multilayers they all had diffusion coefficients of D .apprx. 10-8 cm2/s independent of chain length. In mixed-phase multilayers, each Cn diI showed mobile fraction which reflected its phase-partition preference.