Glucose 6 Phosphate Dehydrogenase (G6PD) quantitation using biosensors at the point of first contact: a mixed method study in Cambodia.
Glucose 6 Phosphate Dehydrogenase (G6PD) quantitation using biosensors at the point of first contact: a mixed method study in Cambodia.
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DOI：
10.1186/s12936-022-04300-9
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发表时间：
2022-10-04
期刊：
影响因子：
3
通讯作者：
von Seidlein, Lorenz
中科院分区：
文献类型：
--
作者：
Adhikari, Bipin;Tripura, Rupam;Dysoley, Lek;Callery, James J.;Peto, Thomas J.;Heng, Chhoeun;Vanda, Thy;Simvieng, Ou;Cassidy-Seyoum, Sarah;Ley, Benedikt;Thriemer, Kamala;Dondorp, Arjen M.;von Seidlein, Lorenz
葡萄糖-6-磷酸脱氢酶（G6PD）酶活性的定量测定对于决定间日疟疾的适当治疗和提供根治方案至关重要。生物传感器是测量G6PD酶活性的即时半定量分析仪。这项研究的主要目的是评估柬埔寨农村疟疾工作人员（VMWs）在一年多的时间里部署生物传感器的操作方面。在Kravanh转诊医院进行初步指导和培训后，向每位VMW （n = 28）和实验室技术人员（n = 5）提供了一个生物传感器（韩国标准SD生物传感器）和日常使用用品。在接下来的12个月里，vmw每个月都会召开会议进行进修培训，收集物资，并重新校准和测试他们的生物传感器。一份定量的自我管理问卷用于评估初始培训后使用生物传感器所需的技能。随后，访问志愿护士的工作地点，使用观察员管理的问卷进行实地观察和评价。所有基于定量问卷的数据进行描述性分析。在所有参与者中进行了半结构化访谈（ssi），以探讨他们在现场使用生物传感器的经验和实用性。对ssi进行转录和翻译，并进行专题分析。共有33名参与者完成了培训，随后在社区中使用了生物传感器。定量评估表明，使用生物传感器的技能逐步提高。VMWs表示有信心和热情在日常工作中使用生物传感器。在首次接触点提供G6PD检测，避免了患者前往卫生中心进行G6PD检测和根治时必须克服的众多障碍。在志愿护士的日常工作中部署生物传感器也被认为是扩大和加强志愿护士作为社区保健提供者作用的机会。VMWs报告了与使用生物传感器有关的实际问题，例如难以使用两个移液器，难以从机器中提取代码芯片，缓冲管底部狭窄。VMWs认为生物传感器在他们的日常工作中是一个实用和有益的工具。在适当的培训和定期监督之后，可以考虑为VMWs提供生物传感器。在首次接触点提供社区间日疟疾管理可能是消除疟疾的关键。在线版本包含补充材料，可在10.1186/s12936-022-04300-9获得。
Quantitative measurement of Glucose-6-Phosphate Dehydrogenase (G6PD) enzyme activity is critical to decide on appropriate treatment and provision of radical cure regimens for vivax malaria. Biosensors are point-of-care semi-quantitative analysers that measure G6PD enzyme activity. The main objective of this study was to evaluate the operational aspects of biosensor deployment in the hands of village malaria workers (VMWs) in Cambodia over a year. Following initial orientation and training at Kravanh Referral Hospital, each VMW (n = 28) and laboratory technician (n = 5) was provided a biosensor (STANDARD SD Biosensor, Republic of Korea) with supplies for routine use. Over the next 12 months VMWs convened every month for refresher training, to collect supplies, and to recalibrate and test their biosensors. A quantitative self-administered questionnaire was used to assess the skills necessary to use the biosensor after the initial training. Subsequently, VMWs were visited at their location of work for field observation and evaluation using an observer-administered questionnaire. All quantitative questionnaire-based data were analysed descriptively. Semi-structured interviews (SSIs) were conducted among all participants to explore their experience and practicalities of using the biosensor in the field. SSIs were transcribed and translated into English and underwent thematic analysis. A total of 33 participants completed the training and subsequently used the biosensor in the community. Quantitative assessments demonstrated progressive improvement in skills using the biosensor. VMWs expressed confidence and enthusiasm to use biosensors in their routine work. Providing G6PD testing at the point of first contact avoids a multitude of barriers patients have to overcome when travelling to health centres for G6PD testing and radical cure. Deploying biosensors in routine work of VMWs was also considered an opportunity to expand and strengthen the role of VMWs as health care providers in the community. VMWs reported practical concerns related to the use of biosensor such as difficulty in using two pipettes, difficulty in extracting the code chip from the machine, and the narrow base of buffer tube. VMWs considered the biosensor a practical and beneficial tool in their routine work. Providing VMWs with biosensors can be considered when followed by appropriate training and regular supervision. Providing community management of vivax malaria at the point of first contact could be key for elimination. The online version contains supplementary material available at 10.1186/s12936-022-04300-9.
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影响因子：
3
作者：
Adhikari B;Awab GR;von Seidlein L
通讯作者：
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