Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array.
Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytometric bead array.
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DOI：
10.3892/ijmm.12.2.193
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发表时间：
2003-08
影响因子：
5.4
通讯作者：
K. Aoe;A. Hiraki;T. Murakami;K. Murakami;K. Makihata;Kazushi Takao;R. Eda;T. Maeda;K. Sugi;Z. Darżynkiewicz;H. Takeyama
中科院分区：
文献类型：
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作者：
K. Aoe;A. Hiraki;T. Murakami;K. Murakami;K. Makihata;Kazushi Takao;R. Eda;T. Maeda;K. Sugi;Z. Darżynkiewicz;H. Takeyama
多种细胞因子在胸腔积液的发生发展中起重要作用。然而，鲜为人知的是，有关可能的相互作用，个别细胞因子在其相对丰度的调节方面的积液。我们研究了国立三洋医院的93例胸腔积液患者（68例男性和25例女性;平均年龄64岁）。22名患者患有结核性胸膜炎，40名患有恶性胸膜炎，31名患者因结核或癌症以外的病因而出现积液（其他）。通过流式微珠阵列（CBA）同时测量IL-2、IL-4、IL-5、IL-10、TNF-α和INF-γ的胸腔液浓度。与其他患者相比，结核性胸膜炎患者的IL-4/IL-5、IL-4/TNF-α、IL-2/TNF-α和IL-10/TNF-α比值较低。在所有三组患者中，在IL-2与IL-4、IL-5、IL-10或TNF-α的丰度之间，在IL-4与IL-10之间，以及在TNF-α与INF-γ之间观察到显著相关性。在恶性胸腔积液患者中，IL-2与IL-4、TNF-α或INF-γ之间，IL-4与INF-γ之间以及TNF-α与INF-γ之间存在显著相关性。在结核性胸腔积液患者中，IL-2与TNF-α、IL-4与IL-10以及TNF-α与INF-γ之间存在显著相关性。在混杂性胸腔积液患者中，IL-2与IL-4、IL-10或TNF-α之间，IL-4与IL-10、TNF-α之间以及IL-10与TNF-α之间存在显著相关性。在其他细胞因子对之间未观察到显著相关性。胸膜液中特定细胞因子之间丰度的强相关性表明它们之间的串扰，就其中一种细胞因子水平的改变提供了调节另一种细胞因子合成和/或分泌的反馈机制而言。这种相互作用可能在积液的发病机制和严重程度中起重要作用。CBA方法提供了一个方便的工具来调查这些相互作用。
Several cytokines play significant roles in the development and pathogenesis of pleural effusion. Little is known, however, about possible interactions between individual cytokines in terms of regulation of their relative abundance in the effusion. We studied 93 patients presenting with pleural effusion to the National Sanyo Hospital (68 men and 25 women; mean age, 64 years). Twenty-two patients had tuberculous pleurisy, 40 had malignant pleuritis, and 31 had effusions due to an etiology other than tuberculosis or cancer (miscellaneous). Pleural fluid concentrations of IL-2, IL-4, IL-5, IL-10, TNF-alpha, and INF-gamma were simultaneously measured by cytometric bead array (CBA). The ratios of IL-4/IL-5, IL-4/TNF-alpha, IL-2/TNF-alpha, and IL-10/TNF-alpha were lower in patients with tuberculosis pleurisy compared with other patients. In all three groups of patients significant correlation was seen between abundance of IL-2 vs. IL-4, IL-5, IL-10, or TNF-alpha, between IL-4 vs. IL-10, and between TNF-alpha vs. INF-gamma. In malignant pleural fluid patients, the significant correlation was between IL-2 vs. IL-4, TNF-alpha, or INF-gamma, between IL-4 vs. INF-gamma, and between TNF-alpha vs. INF-gamma. In tuberculosis pleural fluid patients, the significant correlation was between IL-2 vs. TNF-alpha, between IL-4 vs. IL-10, and between TNF-alpha vs. INF-gamma. In miscellaneous pleural fluid patients, the significant correlation was between IL-2 vs. IL-4, IL-10, or TNF-alpha, between IL-4 vs. IL-10, TNF-alpha, and between IL-10 vs. TNF-alpha. No significant correlation was observed between other pairs of cytokines. Strong correlation in abundance between particular cytokines in pleural fluids suggests cross-talk between them, in terms that an altered level of one of them provides a feedback mechanism regulating synthesis and/or secretion of another one. Such interactions may play important roles in pathogenesis and severity of the effusion. The CBA methodology provides a convenient tool to investigate these interactions.