Genome-Wide Identification and Expression Analysis of theDofGene Family inMedicago sativaL. Under Various Abiotic Stresses
Genome-Wide Identification and Expression Analysis of theDofGene Family inMedicago sativaL. Under Various Abiotic Stresses
复制标题
不同非生物胁迫下紫花苜蓿Dof基因家族的全基因组鉴定及表达分析
DOI：
10.1089/dna.2020.5652
复制
发表时间：
2020-10-02
影响因子：
3.1
通讯作者：
Zhou, Qiang
中科院分区：
文献类型：
--
作者：
Cao, Bo;Cui, Yue;Zhou, Qiang
Dof转录因子是一种植物特异性转录调节因子，在植物发育过程中起重要作用，并在植物的逆境应答中起介导作用。然而，以前已经在几种植物中鉴定出Dofs，但在苜蓿（Medicago sativa L.）最广泛种植的饲料豆类之一。本研究共鉴定了40个MsDof基因，并对这些基因的系统发育重建、分类、保守基序以及在脱落酸（阿坝）、低温、高温、干旱和盐胁迫下的表达模式进行了综合分析。苜蓿Dofgenes家族可分为8个类群。基因本体论（GO）和组织特异性分析表明，大多数MsDof基因可能参与植物生长过程中的生物学功能。此外，表达谱和定量实时PCR分析表明，8个候选的非生物耐受基因诱导响应4个非生物胁迫。本研究在全基因组水平上确定了苜蓿非生物耐性候选基因在抗逆中发挥多种作用的可能性，为苜蓿Dof家族的研究提供了新的信息。
The Dof transcription factor is a plant-specific transcriptional regulator that plays important roles in plant development and acts as a mediator in plant external stress responses. However, Dofs have previously been identified in several plants but not in alfalfa (Medicago sativaL.), one of the most widely cultivated forage legumes. In the present study, a total of 40MsDofgenes were identified, and the phylogenetic reconstruction, classification, conserved motifs, and expression patterns under abscisic acid (ABA), cold, heat, drought and salt stresses of theseDofgenes were comprehensively analyzed. TheDofgenes family in alfalfa could be classified into eight classes. Gene ontology (GO) and tissue-specific analysis indicated that mostMsDofgenes may be involved in biological functions during plant growth. Moreover, the expression profiles and quantitative real-time PCR analysis indicated that eight candidate abiotic tolerance genes were induced in response to four abiotic stresses. This study identified the possibility of abiotic tolerance candidate genes playing various roles in stress resistance at the whole genome level, which would provide new information on the Dof family in alfalfa.