Analogue pigment studies of chromophore-protein interactions in metarhodopsins.
Analogue pigment studies of chromophore-protein interactions in metarhodopsins.
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变视紫红质中发色团-蛋白质相互作用的模拟色素研究。
DOI：
10.1021/bi00428a075
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Crouch,RK
中科院分区：
文献类型：
--
作者：
Renk,G;Crouch,RK
材料和方法除非另有说明，所有实验均在暗红光下进行。吸收光谱在 Varían 2200 光谱仪上记录。Retináis（图 1）。 11-m-Retinal (1) 是霍夫曼-拉罗氏公司的慷慨捐赠。 9-cii-Retinal 购自 Sigma Chemical Co.。11-cis-13-Desmethylretinal (2) 是 Walter Waddell 博士的慷慨捐赠，使用前通过薄层色谱 (TLC) 在 10% 乙醚/己烷中进行纯化。 9-cis-2按照Kropf等人(1973)的方法制备和纯化。 11-4-羟基视黄醛 (3) 由 11-顺式-视黄醛通过在二氧化硒存在下在干燥四氢呋喃中于 80°C 回流 90 分钟合成，并在室温下搅拌过夜 (Renk
Materials and MethodsAll experiments were performed under dim red light except where noted. Absorption spectra were recorded on a Varían 2200 spectrometer.Retináis (Figure 1). 11-m-Retinal (1) was the generous gift of Hoffmann-La Roche. 9-cii-Retinal was purchased from Sigma Chemical Co. 11-cis-13-Desmethylretinal (2) was the generous gift of Dr. Walter Waddell and was purified before use by thin-layer chromatography (TLC) in 10% ether/hex-anes. 9-cis-2 was prepared and purified according to the method of Kropf et al.(1973). 11 j-4-Hydroxyretinal (3) was synthesized from 11-cis-retinal by refluxing in dry tet-rahydrofuran at 80 C inthe presence of selenium dioxide for 90 min and was stirred at room temperature overnight (Renk