β-cyclodextrin as the vehicle for forming ratiometric mercury ion sensor usable in aqueous media, biological fluids, and live cells.
β-cyclodextrin as the vehicle for forming ratiometric mercury ion sensor usable in aqueous media, biological fluids, and live cells.
复制标题
DOI：
10.1021/la103368z
复制
发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Gang Fang;Meiyun Xu;Fang Zeng;Shuizhu Wu
中科院分区：
文献类型：
--
作者：
Gang Fang;Meiyun Xu;Fang Zeng;Shuizhu Wu
具有快速、简便、适用于环境和生物环境的有毒过渡金属离子的选择性、灵敏的检测方法具有重要意义。在这项研究中，我们设计了一种基于β-CD的比率传感器，用于检测水介质、一些生物液和活细胞中的汞离子。在该传感平台中，含有硫代氨基甲酰亚胺的探针染料共价连接到亲水性β-CD环上，有利于与金属离子的络合，而给体染料通过金刚烷基固定在疏水的β-CD腔内，有利于避免自聚集，提高给体染料的量子产率。探针染料与汞离子缔合后，发生开环过程，作为能量受体，与供体染料构成FRET体系，可实现对水中汞离子的比例式检测，检测下限为10 nm。环糊精在传感体系中起着至关重要的作用，它不仅能同时容纳给体染料和探针染料，在汞离子的作用下形成FRET体系，而且使传感器具有水溶性和细胞膜的通透性。该无毒传感平台可用于水介质、生物体液和活细胞(L929和Hela)中汞离子的检测。我们还发现，传感器被L929细胞摄取后，没有显示出细胞毒性，细胞的增殖也没有受到影响。
The selective and sensitive detection methods for toxic transition-metal ions, which are rapid, facile, and applicable to the environmental and biological milieus, are of great importance. In this study, we designed a β-CD-based ratiometric sensor for detecting mercury ions in aqueous media, some biological fluids, and live cells. In this sensing platform, the thiocarbamido-containing probe dye was covalently linked onto the hydrophilic β-CD rim, which is conducive to complexing with metal ion, while the donor dye was anchored inside hydrophobic β-CD cavity via the adamantyl moiety, which is good for avoiding self-aggregation and enhancing the quantum yield of the donor dye. Upon associating with mercury ion, the probe dye undergoes ring-opening process and serves as the energy acceptor and constitutes the FRET system with the donor dye; by this way ratiometric detection of mercury ion in water can be realized with the detection limit of 10 nM. The cyclodextrin plays a crucial role for the sensing system; it not only accommodates both the donor dye and the probe dye which can form FRET system upon addition of Hg(2+) but also makes the sensor water-soluble and cell membrane permeable. This nontoxic sensing platform can be used for mercury ion detection in aqueous medium, biological fluids, and live cells (L929 and Hela). We also found that, upon being taken up by L929 cells, the sensor exhibited no cytotoxicity, and the cell proliferation was not affected.