Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice
Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice
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小鼠皮下白色脂肪组织对急性冷暴露的转录反应
DOI：
10.3390/ijms20163968
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发表时间：
2019-08-02
影响因子：
5.6
通讯作者：
Wang, Yanfang
中科院分区：
文献类型：
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作者：
Liang, Xiaojuan;Pan, Jianfei;Wang, Yanfang
米色脂肪组织被认为在对抗肥胖及其相关代谢疾病方面具有潜在的应用。然而，急性冷刺激米色形成的机制仍然在很大程度上是未知的。在此，进行急性冷刺激（4 ℃持续4小时）皮下白色脂肪组织（sWAT）的转录分析以确定可能参与米色形成的分子特征。组织学分析证实了在急性冷处理的sWAT中出现米色脂肪细胞。RNA测序数据显示714个基因差异表达（p值< 0.05 and fold change >2），其中221个基因上调，493个基因下调。基因本体（GO）分析表明，上调基因富集在与脂质代谢过程、脂肪酸代谢过程、脂质氧化、脂肪酸氧化等相关的GO术语中。相反，下调基因被分配在免疫应答调节、对刺激的应答调节、防御应答、在低温处理的sWAT和3 T3-L1细胞布朗宁分化中验证了部分布朗宁候选基因的表达。总之，我们的研究结果说明了sWAT对急性冷暴露的转录反应，并确定了参与小鼠米色脂肪细胞形成的基因，包括Acad 11，Cyp 2 e1，Plin 5和Pdk 2。
Beige adipose tissue has been considered to have potential applications in combating obesity and its related metabolic diseases. However, the mechanisms of acute cold-stimulated beige formation still remain largely unknown. Here, transcriptional analysis of acute cold-stimulated (4 degrees C for 4 h) subcutaneous white adipose tissue (sWAT) was conducted to determine the molecular signatures that might be involved in beige formation. Histological analysis confirmed the appearance of beige adipocytes in acute cold-treated sWAT. The RNA-sequencing data revealed that 714 genes were differentially expressed (p-value < 0.05 and fold change > 2), in which 221 genes were upregulated and 493 genes were downregulated. Gene Ontology (GO) analyses showed that the upregulated genes were enriched in the GO terms related to lipid metabolic process, fatty acid metabolic process, lipid oxidation, fatty acid oxidation, etc. In contrast, downregulated genes were assigned the GO terms of regulation of immune response, regulation of response to stimulus, defense response, etc. The expressions of some browning candidate genes were validated in cold-treated sWAT and 3T3-L1 cell browning differentiation. In summary, our results illustrated the transcriptional response of sWAT to acute cold exposure and identified the genes, including Acad11, Cyp2e1, Plin5, and Pdk2, involved in beige adipocyte formation in mice.