Ultrabright organic fluorescent microparticles for in vivo tracing applications
Ultrabright organic fluorescent microparticles for in vivo tracing applications
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用于体内示踪应用的超亮有机荧光微粒
DOI：
10.1039/c6tb01782g
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发表时间：
2016
影响因子：
7
通讯作者：
Zhang Hao-Li
中科院分区：
文献类型：
--
作者：
Sun Chun-Lin;Li Ting;Jiang Jian-Qiao;Li Jun;Jiang Dong-Ming;Cao Jing-Jing;Zhang Shengxiang;Zhang Hao-Li
本文报道了荧光有机微粒的体内分布、毒性、代谢及其在脑血流示踪中的应用。荧光微粒具有明亮的荧光，良好的光稳定性和低毒性，因此，它们是理想的长期非侵入性体内示踪。与染色整个血管的常规荧光标记剂相比，示踪剂微粒可以容易地单独跟踪并提供有关血流行为的重要信息。此外，我们在活体动物中观察到这些微粒的受激发射。这些微粒可以提供前所未有的对比度，同时观察血管的分布和微循环的动力学。病理学检查显示，注射的微粒最终聚集在脾脏和肝脏中。我们发现微粒对细胞或小鼠器官没有可观察到的毒性。我们证明，这些荧光微粒适用于非侵入性血流追踪领域的应用，并可以发挥传统的成像剂的补充作用。
We report the in vivo distribution, toxicity and metabolism of micro-sized fluorescent organic particles and their applications in cerebral blood flow tracing. The fluorescent microparticles exhibit bright fluorescence, good photo-stability and low toxicity; therefore, they are ideal for long-term non-invasive in vivo tracing. In contrast to conventional fluorescent labeling agents, which stain the entire blood vessel, the tracer microparticles can be easily tracked individually and provide vital information about blood flow behavior. Furthermore, we observed stimulated emission from these microparticles in living animals. These microparticles can provide unprecedented contrast for simultaneous observation of the distribution of blood vessels and the dynamics of microcirculation. Pathological examination revealed that the injected microparticles eventually collected in the spleen and liver. We found no observable toxicity of the microparticles to cells or mouse organs. We demonstrate that these fluorescent microparticles are suitable for applications in the field of non-intrusive blood flow tracing and could play a complementary role to traditional imaging agents.