Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice
Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice
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DOI：
10.1007/s00425-012-1756-1
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发表时间：
2013-01-01
期刊：
影响因子：
4.3
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Zhou, Kunneng;Ren, Yulong;Wan, Jianmin
叶绿素和叶黄素是植物光合机构中含量最丰富、最基本的两种物质，在植物生长发育中起着重要作用。在这项研究中，我们的特点是一个水稻突变体命名为幼叶失绿症1（ylc 1）从钴-60辐射人口。与野生型相比，ylc 1突变体的幼叶叶绿素和叶黄素含量明显降低，透射电镜分析表明，类囊体片层结构明显疏松。而突变体则逐渐变绿色，在最大分蘖期接近正常绿色。通过图位克隆的方法分离了幼叶失绿症1（YLC 1）基因，并鉴定其编码一种功能未知的蛋白质，属于DUF 3353超家族。互补和RNA干扰试验证实了YLC 1基因的作用，它在所有测试的水稻组织中表达，特别是在叶片中。实时荧光定量PCR分析表明，在不同温度下，与叶绿素合成和光合作用相关的基因的表达水平受到影响。在水稻原生质体中，YLC 1蛋白具有典型的叶绿体定位模式。N-末端50个氨基酸残基被证实是叶绿体靶向所必需和足够的。这些数据表明，YLC 1蛋白可能参与水稻叶片发育早期叶绿素和叶黄素的积累以及叶绿体的发育。
Chlorophyll (Chl) and lutein are the two most abundant and essential components in photosynthetic apparatus, and play critical roles in plant development. In this study, we characterized a rice mutant named young leaf chlorosis 1 (ylc1) from a Co-60-irradiated population. Young leaves of the ylc1 mutant showed decreased levels of Chl and lutein compared to those of wild type, and transmission electron microscopy analysis revealed that the thylakoid lamellar structures were obviously loosely arranged. Whereas, the mutant turns green gradually and approaches normal green at the maximum tillering stage. The Young Leaf Chlorosis 1 (YLC1) gene was isolated via map-based cloning and identified to encode a protein of unknown function belonging to the DUF3353 superfamily. Complementation and RNA-interference tests confirmed the role of the YLC1 gene, which expressed in all tested rice tissues, especially in the leaves. Real-time PCR analyses showed that the expression levels of the genes associated with Chl biosynthesis and photosynthesis were affected in ylc1 mutant at different temperatures. In rice protoplasts, the YLC1 protein displayed a typical chloroplast location pattern. The N-terminal 50 amino acid residues were confirmed to be necessary and sufficient for chloroplast targeting. These data suggested that the YLC1 protein may be involved in Chl and lutein accumulation and chloroplast development at early leaf development in rice.