A glucose-responsive complex polymeric micelle enabling repeated on-off release and insulin protection
A glucose-responsive complex polymeric micelle enabling repeated on-off release and insulin protection
复制标题
葡萄糖响应性复合聚合物胶束，可实现重复开关释放和胰岛素保护
DOI：
10.1039/c2sm26690c
复制
发表时间：
2013-01-01
期刊：
影响因子：
3.4
通讯作者：
Shi, Linqi
中科院分区：
文献类型：
--
作者：
Liu, Gan;Ma, Rujiang;Shi, Linqi
通过两种两嵌段共聚物的自组装制备了葡萄糖响应型复合聚合物胶束(CPM)，即聚乙二醇聚(b-聚天冬氨酸-天冬氨酸苯基硼酸)(PEGb-P(Asp-co-AspPBA))和聚(N-异丙基丙烯酰胺)-b-聚(天冬氨酸-co-天冬氨酸苯基硼酸)(PNIPAM-b-P(Asp-co-AspPBA))。通过控制PNIPAM和聚乙二醇(W-PNIPAM/W-PEG=6/4)的质量比，嵌段共聚物形成了具有新型核-壳-冠结构的复合胶束。通过遵循这种结构，连续的PNIPAM外壳在葡萄糖响应的P(Asp-co-AspPBA)核心上坍塌。结果，CPM对葡萄糖浓度的变化表现出可逆的肿胀，使胰岛素能够重复地开关释放，受葡萄糖水平的调节。此外，CPM还能有效地保护包裹的胰岛素免受酶的降解。因此，这种葡萄糖响应型CPM为构建用于糖尿病治疗的自我调节的胰岛素递送系统提供了一种简单而有效的策略。
We developed a glucose-responsive complex polymeric micelle (CPM) through the self-assembly of two types of diblock copolymers, poly(ethylene glycol)-b-poly(aspartic acid-co-aspartamidophenylboronic acid) (PEG-b-P(Asp-co-AspPBA)) and poly(N-isopropylacrylamide)-b-poly(aspartic acid-co-aspartamidophenylboronic acid) (PNIPAM-b-P(Asp-co-AspPBA)). By controlling the weight ratio between PNIPAM and PEG (W-PNIPAM/W-PEG = 6/4), the block copolymers form complex micelles with a novel core-shell-corona structure. By following this structure, the continuous PNIPAM shell collapsed on the glucose-responsive P(Asp-co-AspPBA) core. As a result, the CPM exhibits a reversible swelling in response to changes in the glucose concentration, enabling the repeated on-off release of insulin regulated by glucose level. Furthermore, the CPM could effectively protect the encapsulated insulin against protease degradation. Therefore, this glucose-responsive CPM provides a simple and powerful strategy to construct a self-regulated insulin delivery system for diabetes treatment.