Specific binding of protein kinase CK2 catalytic subunits to tubulin
Specific binding of protein kinase CK2 catalytic subunits to tubulin
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DOI：
10.1016/s0014-5793(99)01492-1
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发表时间：
1999-11-26
期刊：
影响因子：
3.5
通讯作者：
Montenarh, M
中科院分区：
文献类型：
--
作者：
Faust, M;Schuster, N;Montenarh, M
蛋白激酶CK2由两个调节的β亚基和两个催化的α或α‘亚基组成，为了分别分析这些亚基，我们产生了针对α、α’和β亚基的抗体，用这些抗体进行的免疫荧光研究表明，这三个CK2亚基都存在于细胞质中，弱存在于细胞核中，核膜周围有强信号。双重染色实验显示这三个亚基都与微管蛋白共定位，免疫共沉淀实验和Far Western分析证实了CK2α和α‘亚基与微管蛋白之间的直接联系。不存在CK2β亚基与微管蛋白的结合，因此，我们已经确定了CK2催化亚基的新的结合伙伴，(C)1999欧洲生化学会联合会。
Protein kinase CK2 is composed of two regulatory beta-snbnnits and two catalytic alpha- or alpha'-subunits, To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit, Immunofluorescence studies with these antibodies revealed the presence of all three CK2 subunits in the cytoplasm and weakly in the nucleus with strong signals around the nuclear membrane. Double staining experiments revealed a co-localisation of all three subunits with tubulin, A direct association between the CK2 alpha- and the alpha'-subunit and tubulin was confirmed by coimmunoprecipitation experiments as well as by Far Western analysis. There was no binding of the CK2 beta-subunit to tubulin, Thus, with tubulin we have identified a new binding partner specific for the catalytic subunits of CK2, (C) 1999 Federation of European Biochemical Societies.