Determination of five arsenic species in whole blood by liquid chromatography coupled with inductively coupled plasma mass spectrometry
Determination of five arsenic species in whole blood by liquid chromatography coupled with inductively coupled plasma mass spectrometry
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DOI：
10.1039/c001081b
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发表时间：
2010-01-01
影响因子：
3.4
通讯作者：
Parsons, Patrick J.
中科院分区：
文献类型：
--
作者：
Ito, Kanna;Palmer, Christopher D.;Parsons, Patrick J.
建立了全血中五种砷（As（III）、As（V）、甲基胂酸（MMA）、二甲基胂酸（DMA）和砷甜菜碱（AsB））的液相色谱-电感耦合等离子体质谱（LC-MS）定量分析方法。该方法可用于全血和尿中砷形态的常规监测，但样品制备方法不同。用含有3 mM HgCl（2）的试剂将全血稀释1 + 4，以防止As（III）与LC柱结合。对稀释的血液进行超滤，以去除大于3，000道尔顿的分子。全血中砷的测定在12分钟内完成，尿中砷的测定在11分钟内完成;每种砷的方法检测限< 0.3 μ g/L。通过拟定方法测定的砷物质总和与全血和尿液参比物质以及存档的能力验证材料中总砷的指定值一致。一些尿液参考物质提供了关于砷存在的其他信息，我们的检测结果与文献值吻合良好。
A method was developed to quantify five arsenic species (As(III), As(V), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), and arsenobetaine (AsB)) in whole blood, using liquid chromatography (LC) coupled with inductively coupled plasma mass spectrometry (ICP-MS). The method can be used for routine monitoring of arsenic species in whole blood as well as in urine, but with different sample preparation. Whole blood was diluted 1 + 4 with reagents containing 3 mM HgCl(2), which prevents As(III) from binding to the LC column. The diluted blood was subjected to ultrafiltration so as to remove molecules larger than 3,000 Daltons. Analysis was completed within 12 min, for the determination of arsenic species in whole blood, and within 11 min in urine; the method detection limit is < 0.3 mu g L(-1) for each arsenic species. The sum of arsenic species measured by the proposed method agreed well with assigned values for total arsenic in whole blood and urine reference materials, and in archived proficiency testing materials. Some urine reference materials provided additional information on the arsenic species present, and our testing results agreed well with the documented values.