The Novel Mutant scl of the Medaka Fish, Oryzias latipes, Shows No Secondary Sex Characters
The Novel Mutant scl of the Medaka Fish, Oryzias latipes, Shows No Secondary Sex Characters
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DOI：
10.2108/zsj.25.299
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
Tadashi Sato;A. Suzuki;N. Shibata;M. Sakaizumi;S. Hamaguchi
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文献类型：
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作者：
Tadashi Sato;A. Suzuki;N. Shibata;M. Sakaizumi;S. Hamaguchi
摘要在青冈稻中发现了一个既不具有雄性也不具有雌性第二性征的新突变体。XX和XY成熟突变体都有性腺和许多精子，但当突变体与正常雄性或正常雌性配对时，不会发生产卵。用野生型雌性未受精卵和XY突变体的精子进行人工授精，成功地获得了F1后代。该杂交F_1代未出现突变表型。F_1代杂交产生17个突变后代(25%)，表明该突变表型是由单个隐性突变引起的。这个突变体被命名为scl(无性别特征)。由于雄激素对XY突变体的雄性第二性征乳头突起有诱导作用，因此雄激素受体似乎功能正常。我们在突变体的P450c17基因中发现了一种功能失调型突变；该基因编码一种类固醇生成酶，该酶是产生雌激素和雄激素所必需的。SCL表型与P450c17突变表型完全连锁，强烈提示P450c17基因突变与scl突变表型有关。
Abstract A new mutant that has neither male nor female secondary sex characters was found in the medaka, Oryzias latipes. Both XX and XY mature mutants had gonads with many spermatozoa, but spawning did not occur when the mutants were paired with normal males or normal females. F1 progeny were successfully obtained by artificial insemination using unfertilized eggs from wild-type females and spermatozoa of the XY mutant. The mutant phenotype did not occur in the F1 progeny from this cross. Incrossing among the F1 progeny produced 17 mutant offspring out of 68 progeny (25%), demonstrating that the mutant phenotype is caused by a single recessive mutation. This mutant was named scl (sex character-less). Because papillary processes, a male secondary sex character, were induced in the XY mutants by androgen administration, it seems that the androgen receptor is functioning normally. We found a loss-of-function type mutation in the P450c17 gene of the mutant; this gene encodes a steroidogenic enzyme required for the production of estrogen and androgen. The scl phenotype was completely linked to the mutant genotype of P450c17, strongly suggesting that mutation at the P450c17 locus is responsible for the scl mutant phenotype.