Analysis of the potential for pancreatic cancer metastasis in vitro and in vivo.
Analysis of the potential for pancreatic cancer metastasis in vitro and in vivo.
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DOI：
10.1007/978-1-62703-287-2_17
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Xie, Keping
中科院分区：
文献类型：
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作者：
Huang, Chen;Xie, Keping
胰腺癌仍然是一个具有挑战性的疾病，总体5年生存率低于5%，主要原因是它具有极高的侵袭和转移潜力。这种可能性可能导致这样一个事实，即在超过四分之三的胰腺癌患者中，它已经扩散到局部和远处器官，排除了根治性切除。因此，迫切需要进一步了解胰腺癌转移的机制。在这一章中，我们描述了我们的方法来确定胰腺癌细胞的转移潜力。具体来说，我们报告这些方法的细节，包括在体外测定的迁移，侵袭，粘附，血管生成和体内模型的肝和肺转移和血管生成。
Pancreatic cancer remains a challenging disease, with an overall 5-year survival rate below 5%, the main reason being that it has an extremely high potential for invasion and metastasis. This potential may contribute to the fact that in more than three fourths of patients diagnosed with pancreatic cancer, it has already spread locally and to distant organs, precluding curative resection. Therefore, improved understanding of the mechanisms underlying pancreatic cancer metastasis is urgently needed. In this chapter, we describe our approaches to determining the metastatic potential of pancreatic cancer cells. Specifically, we report the details of these approaches, including in vitro assays of migration, invasion, adhesion, and angiogenesis and in vivo models of liver and lung metastasis and angiogenesis.