Batrachochytrium dendrobatidis gen et sp nov, a chytrid pathogenic to amphibians
Batrachochytrium dendrobatidis gen et sp nov, a chytrid pathogenic to amphibians
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DOI：
10.2307/3761366
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发表时间：
1999-03-01
期刊：
影响因子：
2.8
通讯作者：
Nichols, DK
中科院分区：
文献类型：
--
作者：
Longcore, JE;Pessier, AP;Nichols, DK
最近，来自北美、中美和澳大利亚的圈养和野生青蛙死于壶菌属真菌的表皮感染。我们从华盛顿，华盛顿特区国家动物园的一只圈养的蓝色箭毒蛙中分离到一种壶菌纯培养物。使用该分离物，我们在营养琼脂上拍摄了发育阶段，用透射电子显微镜检查了游动孢子，并接种了测试蛙。无盖壶菌单中心或集群发育，具线状假根，假根由单个或多个区域的游动孢子囊发育而成。游动孢子的动体区的重要分类特征表明，该壶菌属壶菌目，但与透射电镜下研究的其他壶菌不同。微管根的特征是，微管根始于动体三联体9-1，平行于动体延伸到核糖体的集合体中。组织学检查的测试青蛙显示，纯培养物感染皮肤的测试青蛙，而皮肤的控制青蛙保持无感染。该真菌被描述为Batrachochytrium dendrobaerugen.et sp. nov.。
Captive and wild frogs from North and Central America and Australia recently have died with epidermal infections by chytridiomycete fungi. We isolated a chytridiomycete into pure culture from a captive, blue poison dart frog that died at the National Zoological Park in Washington, D.C. Using this isolate, we photographed developmental stages on nutrient agar, examined zoospores with transmission electron microscopy, and inoculated test frogs. This inoperculate chytrid develops either monocentrically or colonially and has thread-like rhizoids that arise from single or multiple areas on die developing zoo-sporangium. The taxonomically important features of the kinetosomal region of the zoospore indicate that this chytrid is a member of the Chytridiales but differs from other chytrids studied with transmission electron microscope Its microtubule root, which begins at kinetosome triplets 9-1 and extends parallel to the kinetosome into the aggregation of ribosomes, is distinctive. Histologic examination of test frogs revealed that the pure culture infected the skin of test frogs, whereas the skin of control frogs remained free of infection. The fungus is described as Batrachochytrium dendrobatidis gen. et sp. nov.