Nano-morphology, crystallinity and surface potential of anatase on micro-arc oxidized titanium affect its protein adsorption, cell proliferation and cell differentiation
Nano-morphology, crystallinity and surface potential of anatase on micro-arc oxidized titanium affect its protein adsorption, cell proliferation and cell differentiation
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微弧氧化钛上锐钛矿的纳米形态、结晶度和表面电位对蛋白质吸附、细胞增殖和细胞分化的影响
DOI：
10.1016/j.msec.2019.110204
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发表时间：
2020-02-01
影响因子：
7.9
通讯作者：
Chen, Wen-Cheng
中科院分区：
文献类型：
--
作者：
Lin, Dan-Jae;Fuh, Lih-Jyh;Chen, Wen-Cheng
采用微波辅助水热法（MW）在微弧氧化（MAO）钛表面制备纳米锐钛矿。采用原子力显微镜、开尔文探针力显微镜、拉曼光谱仪和x射线光电子能谱等方法，研究了在MW介质中Ca/P离子浓度和pH对锐钛矿的形貌、结晶度和表面电位的影响。MWDD（在DD水中处理）和MWCP（在含有Ca/P离子的中性pH介质中处理）的表面覆盖有锐钛矿尖刺，这增强了它们的纳米粗糙度和亲水性，并且具有比其他基团更低的表面电位。MWCP9和MWCP11（在pH 9或pH 11含Ca/P离子的介质中处理）表面的纳米沉淀物主要为无定形锐钛矿，P离子较少。表面电位较低的MWDD和MWCP组对带负电荷的牛血清白蛋白的蛋白质吸附性较高。带正电的组蛋白在MWCP11表面的吸附量高于其他组。用ALP和骨桥蛋白测定共培养1、7、14 d的D1小鼠骨髓间充质干细胞的成骨特性。虽然MW组显示D1小鼠骨髓间充质干细胞的活力与MAO组相当，但其优越的亲水性和更高的蛋白质吸附性，从而调节了成骨干细胞的分化，在7天和14天后显示出更高的ALP和骨桥蛋白分泌。纳米级的形貌、结晶度和表面电位改变了MW处理过的钛表面的亲水性和蛋白质吸附，从而调节体外成骨干细胞的分化。
Using a novel microwave-assisted hydrothermal (MW) process we created nano-scale anatase on micro-arc-oxidized (MAO) titanium surface. The morphology/crystallinity and surface potential of the anatase which altered by Ca/P ions concentrations and pH in the MW medium were characterized by atomic force microscopy, Kelvin probe force microscopy, Raman spectrometer, and x-ray photoelectron spectroscopy. The surface of MWDD (processed in DD water) and MWCP (in neutral pH medium with Ca/P ions) are covered with anatase spikes, which enhance their nano-roughness, hydrophilicity, and possess lower surface potential than other groups. The nano-precipitates on surface of MWCP9 and MWCP11 (processed in medium containing Ca/P ions at pH 9 or pH 11) were mainly amorphous anatase with less P ions. The protein adsorption of negatively charged bovine serum albumin was higher in MWDD and MWCP groups which possess lower surface potential. The adsorption of positively charged histones on the surface of MWCP11 was higher compared to the other groups. The osteogenic characterizations of D1 mice bone marrow mesenchymal stem cells co-cultured for 1, 7, and 14 days were measured by ALP and osteopontin assays. Although MW groups revealed comparable viability of D1 mice bone marrow mesenchymal stem cells to MAO group, their superior hydrophilicity and higher protein adsorption, thus regulating the differentiation of osteoprogenitor stem cells demonstrating higher ALP and osteopontin secretion after 7 days and 14 days. The nanoscale topography, crystallinity and surface potential change the hydrophilicity and protein adsorption on the MW treated titanium surface, thus regulating the differentiation of osteoprogenitor stem cells in vitro.