Volumetric structured illumination microscopy enabled by a tunable-focus lens.
Volumetric structured illumination microscopy enabled by a tunable-focus lens.
复制标题
DOI：
10.1364/ol.40.004943
复制
发表时间：
2015-11-01
期刊：
影响因子：
3.6
通讯作者：
Maitland KC
中科院分区：
文献类型：
--
作者：
Hinsdale T;Malik BH;Olsovsky C;Jo JA;Maitland KC
提出了一种无机械扫描的生物组织体积成像方法。通过结构化照明提供光学切片，并且使用电可调焦距透镜改变成像平面的深度。我们的特点和评估的能力，这种轴向扫描机制的结构照明显微镜，并证明其能够在口腔粘膜离体进行亚细胞分辨率成像。所提出的机制可以潜在地将任何宽视场显微镜转换为3D成像平台，而不需要对成像光学器件和/或样品进行机械扫描。
We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.