MOLECULAR-CLONING OF A HUMAN GENOMIC REGION CONTAINING THE H-BLOOD-GROUP ALPHA(1,2)FUCOSYLTRANSFERASE GENE AND 2 H-LOCUS-RELATED DNA RESTRICTION FRAGMENTS - ISOLATION OF A CANDIDATE FOR THE HUMAN SECRETOR BLOOD-GROUP LOCUS
MOLECULAR-CLONING OF A HUMAN GENOMIC REGION CONTAINING THE H-BLOOD-GROUP ALPHA(1,2)FUCOSYLTRANSFERASE GENE AND 2 H-LOCUS-RELATED DNA RESTRICTION FRAGMENTS - ISOLATION OF A CANDIDATE FOR THE HUMAN SECRETOR BLOOD-GROUP LOCUS
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DOI：
10.1074/jbc.270.9.4632
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发表时间：
1995-03-03
影响因子：
4.8
通讯作者：
GIORGI, D
中科院分区：
文献类型：
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作者：
ROUQUIER, S;LOWE, JB;GIORGI, D
我们使用人H血型α（1，2）岩藻糖基转移酶（FUT 1）cDNA筛选19号染色体粘粒文库以寻找人分泌（Se）血型基因（FUT 2）。我们组装了一个100-kb的粘粒重叠群，定位于19 q13，3，包含FUT 1和两个FUT 1相关序列，称为Sec 1和Sec 2，对于分泌子候选者1和2，Sec 1和Sec 2相隔12 kb，分别与FUT 1基因相距65.5 kb和35 kb，我们使用了粘粒依赖的直接cDNA选择方法来克隆对应于源自Sec 2的转录本的cDNA。该cDNA检测已知表达Se基因座的人组织中的3.35-kb转录物，以及随附文章中报道的序列和表达数据（Kelly，R，J.，Rouquier，S.，Giorgi，D.，Lennon，G. G.，和Lowe，J. B.（1995）J.Biol.Chem.270，4640-4649），这些数据证明Sec 2对应于人Se血型基因座（FUT 2）。我们的结果进一步确定了LT和Se基因座之间的物理关系，并证实了这两个基因座代表不同但紧密连锁的α（1，2）岩藻糖基转移酶基因的假设
We have used the human H blood group alpha(1,2)fucosyltransferase (FUT1) cDNA to screen chromosome 19 cosmid libraries in a search for the human Secretor (Se) blood group gene (FUT2), One cosmid has been isolated that contains two distinct segments that cross-hybridize with FUT1. We have assembled a 100-kilobase (kb) cosmid contig, localized to 19q13,3, encompassing FUT1 and the two FUT1-related sequences, termed Sec1 and Sec2, for Secretor candidate 1 and 2, Sec1 and Sec2 are separated by 12 kb and are 65.5 kb and 35 kb apart, respectively, from the FUT1 gene, We used a cosmid-dependent direct cDNA selection method to clone a cDNA corresponding to a transcript that emanates from Sec2. This cDNA detects a 3.35-kb transcript in human tissues known to express the Se locus, Together with sequence and expression data reported in the accompanying article (Kelly, R, J., Rouquier, S., Giorgi, D., Lennon, G. G., and Lowe, J. B. (1995) J. Biol. Chem. 270, 4640-4649), these data demonstrate that Sec2 corresponds to the human Se blood group locus (FUT2), Our results further more define the physical relationship between the LT and Se loci and confirm a hypothesis that these two loci represent distinct but closely linked alpha(1,2)fucosyltransferase genes