MONOCLONAL-ANTIBODIES THAT INHIBIT PLASMODIUM-FALCIPARUM INVASION IN-VITRO RECOGNIZE THE 1ST GROWTH FACTOR-LIKE DOMAIN OF MEROZOITE SURFACE PROTEIN-1
MONOCLONAL-ANTIBODIES THAT INHIBIT PLASMODIUM-FALCIPARUM INVASION IN-VITRO RECOGNIZE THE 1ST GROWTH FACTOR-LIKE DOMAIN OF MEROZOITE SURFACE PROTEIN-1
复制标题
DOI：
10.1016/0166-6851(93)90141-j
复制
发表时间：
1993-08-01
影响因子：
1.5
通讯作者：
HOLDER, AA
中科院分区：
文献类型：
--
作者：
CHAPPEL, JA;HOLDER, AA
在恶性疟原虫的侵入阶段的表面上发现的主要蛋白质，裂殖子表面蛋白-1（MSP 1），已被提议作为候选疫苗。识别该分子（MSP 1（19））的单个片段（由与表皮生长因子（EGF）相关的2个区域组成）的抗体也可在体外抑制寄生虫生长。通过在大肠杆菌中直接表达单个EGF样结构域，表明第一个结构域是生长抑制抗体的靶标。单个氨基酸差异影响一些抗体与该结构域的结合。
A major protein found on the surface of the invasive stage of the malaria parasite Plasmodium falciparum, merozoite surface protein-1 (MSP1), has been proposed as a vaccine candidate. Antibodies which recognise a single fragment of this molecule (MSP1(19)), composed of 2 regions related to epidermal growth factor (EGF), also inhibit parasite growth in vitro. It is shown by direct expression of the individual EGF-like domains in Escherichia coli, that the first domain is the target of growth-inhibitory antibodies. A single amino acid difference influences the binding of some antibodies to this domain.