Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus
Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus
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猪氨肽酶 N 是 PEDV 冠状病毒的功能受体
DOI：
10.1016/j.virol.2007.03.031
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发表时间：
2007-08-15
期刊：
影响因子：
3.7
通讯作者：
Li, Y. J.
中科院分区：
文献类型：
--
作者：
Li, B. X.;Ge, J. W.;Li, Y. J.
猪流行性腹泻病毒（PEDV）引起仔猪致命腹泻，给东亚地区造成巨大经济损失。据报道，氨肽酶 N (APN) 是传染性胃肠炎病毒 (TGEV)、人类冠状病毒 229E (HCoV-229E) 和猫冠状病毒 (FeCoV) 的受体，这些病毒都属于 I 类冠状病毒，包括 PEDV。此前还证实猪氨肽酶N（pAPN）可以与PEDV结合，而抗pAPN抗体可能会抑制这种结合。为了研究 pAPN 是否是 PEDV 的受体，我们用猪氨肽酶 (pAPN) cDNA 转染 MDCK 细胞，这使得非易感细胞能够支持 PEDV 复制和连续病毒传播。此外，感染被针对 pAPN 的抗体阻断，这表明 pAPN 在病毒进入过程中发挥着关键作用。此外，用于检测 pAPN 和 PEDV 抗原的免疫荧光测定，以及使用抗 pAPN 抗体的中和测定，进一步证实了 pAPN 转染的 MDCK 细胞中 pAPN 表达与病毒复制之间的相关性。这些结果表明 pAPN 是 PEDV 的功能性受体。 (c) 2007 Elsevier Inc. 保留所有权利。
Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets that leads to great economic losses in East Asia. It was reported that aminopeptidase N (APN) is the receptor for transmissible gastroenteritis virus (TGEV), human coronavirus 229E (HCoV-229E) and feline coronavirus (FeCoV) which all belong to group I coronavirus including as well as PEDV. It was also confirmed previously that porcine aminopeptidase N (pAPN) can bind to PEDV, and anti-pAPN antibodies may inhibit the combination. To investigate whether pAPN is a receptor for PEDV, we transfected MDCK cells with porcine aminopeptidase (pAPN) cDNA and this enabled non-susceptible cells to support PEDV replication and serial viral propagation. Moreover, the infection was blocked by antibodies against pAPN, implies the critical role of pAPN during virus entry. In addition, immunofluorescence assays for detection of pAPN and PEDV antigens, together with neutralization assays using antibodies against pAPN, further confirmed the correlation between pAPN expression and viral replication in pAPN-transfected MDCK cells. These results indicate that pAPN is a functional receptor for PEDV. (c) 2007 Elsevier Inc. All rights reserved.