Stage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparum
Stage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparum
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DOI：
10.1016/j.trstmh.2004.11.014
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发表时间：
2005-07-01
影响因子：
2.2
通讯作者：
White, NJ
中科院分区：
文献类型：
--
作者：
Desakorn, V;Dondorp, AM;White, NJ
由于它们在微循环中被隔离，恶性疟原虫的致病晚期在恶性疟原虫疟疾患者的外周血样品中代表性不足。寄生虫的排泄物可能有助于估计这种隔离的生物量。我们定量的阶段依赖性生产和释放每个寄生虫的恶性疟原虫富组氨酸蛋白2（PfHRP 2）的目的是测量隔离的生物量。一个简单的方法，有关寄生虫阶段寄生虫年龄的发展，以促进这一点。四株恶性疟原虫感染红细胞PfHRP_2含量的中位数（范围）为2.0fg（0.5-4.3fg），为2.1- 10.2fg（5.4fg）。PfHRP 2在红细胞中的含量在发育至成熟滋养体阶段增加最多。每个寄生虫在整个红细胞周期分泌的PfHRP 2的中位数（范围）为5.2 fg（1.1-13.0 fg）。总PfHRP 2的中位数为89%，排泄时的瞬间，线粒体破裂。这种阶段依赖性PfHRP 2释放的评估是未来研究的必要前提，旨在从外周血PfHRP 2浓度估计总患者寄生虫质量。(c)2005年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
Because of their sequestration in the microcirculation, the pathogenic late stages of Plasmodium falciparum are under-represented in peripheral blood samples from patients with falciparum malaria. Excreted products of the parasite might help to estimate this sequestered biomass. We quantified the stage-dependent production and release per parasite of P falciparum histidine-rich protein 2 (PfHRP2) with the objective of measuring the sequestered biomass. A simple method to relate parasite stage to parasite age was developed to facilitate this. In four isolates of P falciparum, the median (range) PfHRP2 content was 2.0 fg (0.5-4.3fg) for a young ring stage infected erythrocyte, and 5.4 fg (2.1-10.2 fg) for the schizont stage. The amount of PfHRP2 in the parasitized erythrocyte increased most during development to the mature trophozoite stage. The median (range) amount of PfHRP2 secreted per parasite per entire erythrocytic cycle was 5.2 fg (1.1-13.0 fg). A median of 89% of the total PfHRP2 was excreted at the moment of schizont rupture. This assessment of the stage-dependent release of PfHRP2 is an essential prerequisite for future studies aimed at estimating the total patient parasite mass from the peripheral blood PfHRP2 concentration. (c) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.