Programmed regulation of rat offspring adipogenic transcription factor (PPARγ) by maternal nutrition.
Programmed regulation of rat offspring adipogenic transcription factor (PPARγ) by maternal nutrition.
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DOI：
10.1017/s2040174415001440
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发表时间：
2015-12
影响因子：
1.7
通讯作者：
Ross MG
中科院分区：
文献类型：
--
作者：
Desai M;Jellyman JK;Han G;Lane RH;Ross MG
我们测定了脂肪形成转录因子、过氧化物酶体增殖物激活受体γ（过氧化物酶体增殖物激活受体γ）及其辅抑制因子和辅激活因子复合物在营养不良和营养过度的肥胖后代脂肪组织中的蛋白表达。雌性大鼠在交配前以及整个妊娠和哺乳期（Mat-OB）喂食高脂肪（60% kcal）或对照（10% kcal）饲料。从妊娠第10天至足月，其他母鼠的食物限制率为50%（宫内生长受限; IUGR）。在新生和成年雄性后代中分析脂肪组织蛋白表达。正常出生体重的Mat-OB和低出生体重的IUGR新生儿上调了PPARγ，辅抑制因子和辅激活因子发生了不同的变化。作为肥胖成年人，Mat-OB和IUGR后代的PPARγ表达增加，辅抑制因子表达减少，辅激活因子表达增加。在新生儿和成年后代中，营养程序性增加的PPARγ表达与其辅助调节因子的表达改变相关。为了确定其在PPARγ介导的程序性肥胖中的作用，有必要对PPAR γ辅助调节因子进行功能研究。
We determined the protein expression of adipogenic transcription factor, peroxisome proliferator-activated receptor gamma (PPARγ) and its co-repressor and co-activator complexes in adipose tissue from the obese offspring of under- and over-nourished dams. Female rats were fed either a high fat (60% kcal) or control (10% kcal) diet prior to mating, and throughout pregnancy and lactation (Mat-OB). Additional dams were 50% food-restricted from pregnancy day 10 to term (intrauterine growth-restricted; IUGR). Adipose tissue protein expression was analyzed in newborn and adult male offspring. Normal birth weight Mat-OB and low birth weight IUGR newborns had upregulated PPARγ with variable changes in co-repressors and co-activators. As obese adults, Mat-OB and IUGR offspring had increased PPARγ with decreased co-repressor and increased co-activator expression. Nutritionally programmed increased PPARγ expression is associated with altered expression of its co-regulators in the newborn and adult offspring. Functional studies of PPARγ co-regulators are necessary to establish their role in PPARγ-mediated programmed obesity.