Systematic design of cell membrane coating to improve tumor targeting of nanoparticles.
Systematic design of cell membrane coating to improve tumor targeting of nanoparticles.
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细胞膜涂层的系统设计以提高纳米粒子的肿瘤靶向性
DOI：
10.1038/s41467-022-33889-3
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发表时间：
2022-10-19
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞膜（CM）涂层技术越来越多地应用于纳米医学，但整个涂层过程，包括吸附，破裂和融合还没有完全理解。以前，我们发现大多数仿生纳米颗粒（NPs）只是部分包覆，但这种部分包覆的机制仍然不清楚，这阻碍了包覆技术的进一步改进。在这里，我们表明，部分涂层是一个中间状态，由于CM片段或CM囊泡的吸附，后者最终可能会在外力下破裂。由于膜的流动性有限，这种部分涂层难以自我修复以实现完全涂层。基于我们对详细涂层过程的理解，我们开发了一种固定部分CM涂层的一般方法：引入外部磷脂作为辅助物以增加CM流动性，促进脂质贴片的最终融合。用这种方法包被的NP具有高的完全包被率（~23%），并且与传统包被的NP（~6%的完全包被率）相比表现出增强的肿瘤靶向能力。我们的研究结果为固定部分CM涂层以增强肿瘤蓄积提供了机制基础。通过细胞膜（CM）包覆对纳米颗粒进行表面修饰以改善其生物界面性质通常会导致部分包覆。在本文中，作者表明，部分包被是由于CM片段或囊泡吸收而导致的中间状态，并且可以通过使用外部磷脂增加CM流动性来解决。
Cell membrane (CM) coating technology is increasingly being applied in nanomedicine, but the entire coating procedure including adsorption, rupture, and fusion is not completely understood. Previously, we showed that the majority of biomimetic nanoparticles (NPs) were only partially coated, but the mechanism underlying this partial coating remains unclear, which hinders the further improvement of the coating technique. Here, we show that partial coating is an intermediate state due to the adsorption of CM fragments or CM vesicles, the latter of which could eventually be ruptured under external force. Such partial coating is difficult to self-repair to achieve full coating due to the limited membrane fluidity. Building on our understanding of the detailed coating process, we develop a general approach for fixing the partial CM coating: external phospholipid is introduced as a helper to increase CM fluidity, promoting the final fusion of lipid patches. The NPs coated with this approach have a high ratio of full coating (~23%) and exhibit enhanced tumor targeting ability in comparison to the NPs coated traditionally (full coating ratio of ~6%). Our results provide a mechanistic basis for fixing partial CM coating towards enhancing tumor accumulation. Surface modification of nanoparticles by cell membrane (CM) coating to improve their bio-interface properties often results in partial coating. Here the authors show that partial coating is an intermediate state due to the absorption of CM fragments or vesicles and can be resolved by increasing CM fluidity with external phospholipids.
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DOI：
10.1002/adma.202001808
发表时间：
2020-07
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Jiang Y;Krishnan N;Zhou J;Chekuri S;Wei X;Kroll AV;Yu CL;Duan Y;Gao W;Fang RH;Zhang L
通讯作者：
Zhang L
使用加速器质谱法确定体内SIO2纳米颗粒的药代动力学和长期生物分布。
影响因子：
10.8
作者：
Malfatti MA;Palko HA;Kuhn EA;Turteltaub KW
通讯作者：
Turteltaub KW
影响因子：
3.9
作者：
Kure, Tomoko;Sakai, Hiromi
通讯作者：
Sakai, Hiromi
影响因子：
6.3
作者：
Desplat, JC;Pagonabarraga, I;Bladon, P
通讯作者：
Bladon, P
影响因子：
17.1
作者：
Corbo, Claudia;Molinaro, Roberto;Tasciotti, Ennio
通讯作者：
Tasciotti, Ennio