Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics
Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics
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DOI：
10.1038/s41587-020-00794-3
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发表时间：
2021-02-11
影响因子：
46.9
通讯作者：
Jaschke, Andres
中科院分区：
文献类型：
--
作者：
Sunbul, Murat;Lackner, Jens;Jaschke, Andres
发光RNA适配体使单分子定位显微镜的分辨率不受photobleaching.Overcoming以前的荧光发光RNA适配体的超分辨率imaging的局限性，我们提出RhoBAST，一个适配体，结合荧光罗丹明染料快速协会和解离动力学。它的间歇性荧光发射使单分子定位显微镜的分辨率不受光漂白的限制。我们使用RhoBAST对活细胞和固定细胞中RNA的亚细胞结构进行成像，具有约10 nm的定位精度和高信噪比。
A light-up RNA aptamer enables single-molecule localization microscopy with a resolution not limited by photobleaching.Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization microscopy with a resolution not limited by photobleaching. We use RhoBAST to image subcellular structures of RNA in live and fixed cells with about 10-nm localization precision and a high signal-to-noise ratio.