Two Distinct Localization Patterns of Testis-Specific Serine Protease 1 (TESSP1) in the Seminiferous Tubules of the Mouse Testis
Two Distinct Localization Patterns of Testis-Specific Serine Protease 1 (TESSP1) in the Seminiferous Tubules of the Mouse Testis
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DOI：
10.2108/zsj.26.294
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发表时间：
2009-04
影响因子：
9.5
通讯作者：
Naoharu Takano;A. Kimura;Takayuki Takahashi
中科院分区：
文献类型：
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作者：
Naoharu Takano;A. Kimura;Takayuki Takahashi
小鼠Tessp 1已被证明是一个睾丸特异性基因，可能有助于精子发生。在这份报告中，我们提出了一个针对TESSP 1的特异性抗体，以评估其生物学作用。Western blotting检测睾丸TESSP 1在所有出生后发育阶段的小鼠。使用缺乏生殖细胞的W/Wv小鼠的睾丸进行的实验表明，TESSP 1在支持细胞和间质细胞中表达。在野生型小鼠睾丸的免疫荧光染色中，在生精小管的近腔室中观察到TESSP 1的点状信号，而在基底室中发现扩散信号。通常，斑点状和弥散信号分别与跨高尔基体网络标记物RAB 6和跨膜蛋白CADHERIN 2重叠。还观察到与睾丸间质Leydig细胞相关的一些TESSP 1染色。本研究的结果表明，TESSP 1主要定位于质膜的精原细胞和支持细胞的基底室，但表现出细胞内定位，大概在高尔基体，精母细胞和精子细胞的曲细精管的近腔室。TESSP 1在生殖细胞和体细胞中的表达以及在精子发生过程中其在生殖细胞中的细胞定位的改变表明其可能在睾丸中具有独特的作用。
Mouse Tessp1 has been shown to be a testis-specific gene that may contribute to spermatogenesis. In this report, we raised a specific antibody against TESSP1 to assess its biological role. Western blotting detected testicular TESSP1 in all postnatal developmental stages of the mouse. Experiments using the testes of W/Wv mice, which lack germ cells, indicated TESSP1 expression in Sertoli cells and Leydig cells. In immunofluorescence staining of the wild-type mouse testis, dot-like signals for TESSP1 were observed in the adluminal compartment of the seminiferous tubules, while diffused signals were found in the basal compartment. Generally, the dot-like and diffused signals overlapped with the trans-Golgi network marker RAB6 and the transmembrane protein CADHERIN 2, respectively. Some TESSP1 staining was also observed in association with interstitial Leydig cells of the testis. The results of this study suggest that TESSP1 is predominantly localized in the plasma membrane of spermatogonia and Sertoli cells in the basal compartment, but exhibits an intracellular localization, presumably in the Golgi apparatus, of spermatocytes and spermatids in the adluminal compartment of the seminiferous tubules. The expression of TESSP1 in both germ cells and somatic cells and alteration in its cellular localization in the germ cells during spermatogenesis indicate that it may have a unique role in the testis.